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dc.contributor.authorPatel, N.
dc.contributor.authorDykeman, E.C.
dc.contributor.authorCoutts, Robert H.A.
dc.contributor.authorLomonossoff, G.P.
dc.contributor.authorRowlands, D.J.
dc.contributor.authorPhillips, S.E.V.
dc.contributor.authorRanson, N.
dc.contributor.authorTwarock, R.
dc.contributor.authorTuma, R.
dc.contributor.authorStockley, P.G.
dc.date.accessioned2015-06-24T08:20:59Z
dc.date.available2015-06-24T08:20:59Z
dc.date.issued2015-02-17
dc.identifier.citationPatel , N , Dykeman , E C , Coutts , R H A , Lomonossoff , G P , Rowlands , D J , Phillips , S E V , Ranson , N , Twarock , R , Tuma , R & Stockley , P G 2015 , ' Revealing the density of encoded functions in a viral RNA ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 112 , no. 7 , pp. 2227-2232 . https://doi.org/10.1073/pnas.1420812112
dc.identifier.issn1091-6490
dc.identifier.otherPURE: 8655898
dc.identifier.otherPURE UUID: 82ff2b22-9971-4752-94ff-545986f948e6
dc.identifier.otherScopus: 84923271402
dc.identifier.urihttp://hdl.handle.net/2299/16076
dc.descriptionNikesh Patel, et al, ‘Revealing the density of encoded functions in a viral RNA’, Proceedings of the National Academy of Sciences of the United States of America (PNAS), Vol. 112 (7): 2227-2232, February 2015, doi: http:dx.doi.org/10. 1073/pnas.1420812112. This article is freely available online through the PNAS open access option.
dc.description.abstractWe present direct experimental evidence that assembly of a single-stranded RNA virus occurs via a packaging signal-mediated mechanism. We show that the sequences of coat protein recognition motifs within multiple, dispersed, putative RNA packaging signals, as well as their relative spacing within a genomic fragment, act collectively to influence the fidelity and yield of capsid self-assembly in vitro. These experiments confirm that the selective advantages for viral yield and encapsidation specificity, predicted from previous modeling of packaging signal-mediated assembly, are found in Nature. Regions of the genome that act as packaging signals also function in translational and transcriptional enhancement, as well as directly coding for the coat protein, highlighting the density of encoded functions within the viral RNA. Assembly and gene expression are therefore direct molecular competitors for different functional folds of the same RNA sequence. The strongest packaging signal in the test fragment, encodes a region of the coat protein that undergoes a conformational change upon contact with packaging signals. A similar phenomenon occurs in other RNA viruses for which packaging signals are known. These contacts hint at an even deeper density of encoded functions in viral RNA, which if confirmed, would have profound consequences for the evolution of this class of pathogensen
dc.language.isoeng
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.rightsOpen
dc.subjectvirus assembly
dc.subjectsingle-molecule fluorescence correlation spectroscopy
dc.subjectsatellite tobacco necrosis virus
dc.subjectpackaging signal
dc.titleRevealing the density of encoded functions in a viral RNAen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2015-02-17
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1073/pnas.1420812112
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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