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        Human papillomavirus is detected in transitional cell carcinoma arising in renal transplant recipients

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        Author
        Husain, Ehab
        Prowse, David M.
        Ktori, Elena
        Shaikh, Taufiq
        Yaqoob, Magdi
        Junaid, Islam
        Baithun, Suhail
        Attention
        2299/16118
        Abstract
        Aims: We investigated the role of human papillomavirus HPV in the development of transitional cell carcinoma TCC arising in renal transplant recipients. Methods: Genomic DNA was extracted from 10 m paraffin embedded sections of five TCCs arising in five renal transplant recipients using the QIAamp DNA mini kit according to the manufacturer's instructions. β-globin PCR was performed to test DNA adequacy. Samples were tested for the presence of HPV DNA by broad spectrum HPV PCR method using non-biotinylated SPF10 primers SPF1A, SPF1B, SPF1C, SPF1D, SPF2B, SPF2D which amplify a short 65 bp fragment. Positive bands were identified on a 3 gel. Positive samples underwent a second HPV PCR and were amplified using biotinylated SPF10 primer set, which amplifies the same 65 bp region of the L1 open reading frame. INNO-LiPA line probe assay was then performed to genotype the samples which uses a reverse hybridisation principle. Results: Four of five TCCs examined were positive for HPV. The high risk HPV16 was detected in three cases whereas in the fourth case an unclassifiable HPV genotype was present. In all DNA samples, β-globin amplification was successful. Conclusions: Our results indicate that HPV and in particular HPV16 may play an aetiological role in the development of TCC in renal transplant patients.
        Publication date
        2009-11-09
        Published in
        Pathology
        Published version
        https://doi.org/10.1080/00313020902756303
        Other links
        http://hdl.handle.net/2299/16118
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        School of Life and Medical Sciences
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