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dc.contributor.authorMao, Xueying
dc.contributor.authorShaw, Greg
dc.contributor.authorJames, Sharon Y.
dc.contributor.authorPurkis, Patricia
dc.contributor.authorKudahetti, Sakunthala C.
dc.contributor.authorTsigani, Theodora
dc.contributor.authorKia, Saname
dc.contributor.authorYoung, Bryan D.
dc.contributor.authorOliver, R. Tim D
dc.contributor.authorBerney, Dan
dc.contributor.authorProwse, David M.
dc.contributor.authorLu, Yong Jie
dc.date.accessioned2015-07-16T23:10:20Z
dc.date.available2015-07-16T23:10:20Z
dc.date.issued2008-05-01
dc.identifier.citationMao , X , Shaw , G , James , S Y , Purkis , P , Kudahetti , S C , Tsigani , T , Kia , S , Young , B D , Oliver , R T D , Berney , D , Prowse , D M & Lu , Y J 2008 , ' Detection of TMPRSS2 : ERG fusion gene in circulating prostate cancer cells ' , Asian Journal of Andrology , vol. 10 , no. 3 , pp. 467-473 . https://doi.org/10.1111/j.1745-7262.2008.00401.x
dc.identifier.issn1008-682X
dc.identifier.otherPURE: 8733462
dc.identifier.otherPURE UUID: 4f1438b9-e6fc-444b-b828-dcc56a1ff2b9
dc.identifier.otherScopus: 42649108742
dc.identifier.otherPubMed: 18385909
dc.identifier.urihttp://hdl.handle.net/2299/16162
dc.descriptionCreative Commons Attribution-NonCommercial-Share Alike 3.0 license (CC BY-NC SA)
dc.description.abstractAim: To investigate the existence of TMPRSS2:ERG fusion gene in circulating tumor cells (CTC) from prostate cancer patients and its potential in monitoring tumor metastasis. Methods: We analyzed the frequency of TMPRSS2: ERG and TMPRSS2:ETV1 transcripts in 27 prostate cancer biopsies from prostatectomies, and TMPRSS2:ERG transcripts in CTC isolated from 15 patients with advanced androgen independent disease using reverse transcription polymerase chain reaction (RT-PCR). Fluorescence in situ hybridization (FISH) was applied to analyze the genomic truncation of ERG, which is the result of TMPRSS2:ERG fusion in 10 of the 15 CTC samples. Results: TMPRSS2: ERG transcripts were found in 44% of our samples, but we did not detect expression of TMPRSS2:ETV1. Using FISH analysis we detected chromosomal rearrangements affecting the ERG gene in 6 of 10 CTC samples, including 1 case with associated TMPRSS2:ERG fusion at the primary site. However, TMPRSS2:ERG transcripts were not detected in any of the 15 CTC samples, including the 10 cases analyzed by FISH. Conclusion: Although further study is required to address the association between TMPRSS2:ERG fusion and prostate cancer metastasis, detection of genomic truncation of the ERG gene by FISH analysis could be useful for monitoring the appearance of CTC and the potential for prostate cancer metastasis.en
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofAsian Journal of Andrology
dc.rightsOpen
dc.subjectCirculating tumor cells
dc.subjectFluorescence in situ hybridization
dc.subjectFusion gene
dc.subjectMetastasis
dc.subjectPolymerase chain reaction
dc.subjectProstate cancer
dc.subjectTMPRSS2:ERG
dc.subjectNephrology
dc.subjectEndocrinology
dc.titleDetection of TMPRSS2 : ERG fusion gene in circulating prostate cancer cellsen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2008-05-01
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1111/j.1745-7262.2008.00401.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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