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dc.contributor.authorWhitmore, A. C.
dc.contributor.authorProwse, David M.
dc.contributor.authorArnold, L. W.
dc.contributor.authorHaughton, G.
dc.identifier.citationWhitmore , A C , Prowse , D M , Arnold , L W & Haughton , G 1989 , ' Ig isotype switching in B lymphocytes : A method for estimating isotype switch frequency in cloned B cell lymphomas ' , International Immunology , vol. 1 , no. 5 , pp. 532-539 .
dc.identifier.otherPURE: 8734679
dc.identifier.otherPURE UUID: 1e5de515-865b-45ac-ad32-635ce412c78b
dc.identifier.otherScopus: 0024428157
dc.identifier.otherPubMed: 2489041
dc.description.abstractWe have developed a method for enumerating the frequency of Ig isotype switching in clones of B cells. The method adapts Poisson statistics to analyze the distribution of amounts of switched isotype produced by multiple subclones of cells and thus enables one to estimate the probability that a single cell will switch isotype in one cell generation. We have applied this method to determine the spontaneous switch frequency of two Ly-1+ B cell lymphomas of B10-H-2aH-4bp/Wts mice. Both CH12.LX and CH27.LX switch from IgM to IgA at very high frequencies (1 - 5 x 10-3 switch events per cell division) and from IgM to IgG at low but detectable frequencies (10-4 - 10-5 switch events per cell division). Cloned IgG variants of CH12.LX switch to IgA at the same frequency as the IgM-producing cells. Bacterial lipopolysaccharide has a strong inhibitory effect on isotype switching by CH12.LX. Possible explanations for the observed preference for switching to IgA are discussed.en
dc.relation.ispartofInternational Immunology
dc.subjectApplied Mathematics
dc.subjectPublic Health, Environmental and Occupational Health
dc.titleIg isotype switching in B lymphocytes : A method for estimating isotype switch frequency in cloned B cell lymphomasen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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