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dc.contributor.authorKanhayuwa, L.
dc.contributor.authorKotta-Loizou, I.
dc.contributor.authorOzkan, S.
dc.contributor.authorGunning, A. Patrick
dc.contributor.authorCoutts, Robert H.A.
dc.date.accessioned2015-08-12T18:15:43Z
dc.date.available2015-08-12T18:15:43Z
dc.date.issued2015-07-21
dc.identifier.citationKanhayuwa , L , Kotta-Loizou , I , Ozkan , S , Gunning , A P & Coutts , R H A 2015 , ' A novel mycovirus from Aspergillus fumigatus contains four unique double-stranded RNAs as its genome and is infectious as dsRNA ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 112 , no. 29 , pp. 9100-9105 . https://doi.org/10.1073/pnas.1419225112
dc.identifier.issn1091-6490
dc.identifier.otherPURE: 8655970
dc.identifier.otherPURE UUID: 9a8f229a-a6ab-47c3-82b5-0ad771c87387
dc.identifier.otherScopus: 84937831247
dc.identifier.urihttp://hdl.handle.net/2299/16208
dc.descriptionThis document is the Accepted Manuscript version of the following article: Lakkhana Kanhayuwa, Ioly Kotta-Loizou, Selin Özkan, A. Patrick Gunning, and Robert H. A. Coutts, 'A novel mycovirus from Aspergillus fumigatus contains four unique dsRNAs as its genome and is infectious as dsRNA', Proceedings of the National Academy of Sciences of the United States of America (PNAS), Vol. 112 (29): 9100-9105; July 2, 2015. The version of record is available online via doi: https://doi.org/10.1073/pnas.1419225112.
dc.description.abstractWe report the discovery and characterization of a double-stranded RNA (dsRNA) mycovirus isolated from the human pathogenic fungus Aspergillus fumigatus, Aspergillus fumigatus tetramycovirus-1 (AfuTmV-1), which reveals several unique features not found previously in positive-strand RNA viruses, including the fact that it represents the first dsRNA (to our knowledge) that is not only infectious as a purified entity but also as a naked dsRNA. The AfuTmV-1 genome consists of four capped dsRNAs, the largest of which encodes an RNA-dependent RNA polymerase (RdRP) containing a unique GDNQ motif normally characteristic of negative-strand RNA viruses. The third largest dsRNA encodes an S-adenosyl methionine–dependent methyltransferase capping enzyme and the smallest dsRNA a P-A-S–rich protein that apparently coats but does not encapsidate the viral genome as visualized by atomic force microscopy. A combination of a capping enzyme with a picorna-like RdRP in the AfuTmV-1 genome is a striking case of chimerism and the first example (to our knowledge) of such a phenomenon. AfuTmV-1 appears to be intermediate between dsRNA and positive-strand ssRNA viruses, as well as between encapsidated and capsidless RNA virusesen
dc.language.isoeng
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America
dc.subjectmicrovirus
dc.subjectinfectious dsRA
dc.subjectprootplast transfection
dc.subjectcapping enzyme
dc.titleA novel mycovirus from Aspergillus fumigatus contains four unique double-stranded RNAs as its genome and is infectious as dsRNAen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
rioxxterms.versionAM
rioxxterms.versionofrecordhttps://doi.org/10.1073/pnas.1419225112
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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