A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens
Rooker, S. J.
Garvey, C. F.
Hall, L. M.
An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.
Published inInsect Molecular Biology
RelationsSchool of Life and Medical Sciences
MetadataShow full item record
Showing items related by title, author, creator and subject.
Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus Jamal, Atif; Bignell, Elaine M.; Coutts, Robert H.A. (2010-10)A new double-stranded RNA (dsRNA) virus designated A. fumigatus chrysovirus (AfuCV), belonging to the family Chrysoviridae, has been identified in the filamentous fungus Aspergillus fumigatus. The virus was detected in ...
Anopheles stephensi Dox-A2 shares common ancestry with genes from distant groups of eukaryotes encoding a 26S proteasome subunit and is in a conserved gene cluster Garvey, C. F.; Malcolm, Colin (2000-06)The sequence of a cloned Anopheles stephensi gene showed 72% inferred amino acid identity with Drosophila melanogaster Dox-A2 and 93% with its putative ortholog in Anopheles gambiae. Dox-A2 is the reported but herein ...
Kozlakidis, Zisis; Hacker, Caroline V.; Bradley, Dominic; Jamal, Atif; Phoon, Xiyu; Webber, Joan; Brasier, Clive M.; Buck, Kenneth W.; Coutts, Robert H.A. (2009-08)The incomplete sequences of two large, 10-12 kbp, double-stranded RNAs (dsRNAs) found in the TW-2 isolate of the saprophytic fungus, Phlebiopsis gigantea (Pg) are reported. Both PgV-TW2 dsRNA1 and dsRNA2 potentially encode ...