A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens
Author
Malcolm, Colin
Bourguet, D.
Ascolillo, A.
Rooker, S. J.
Garvey, C. F.
Hall, L. M.
Pasteur, N.
Raymond, M.
Attention
2299/16727
Abstract
An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.
Publication date
1998-05Published in
Insect Molecular BiologyPublished version
https://doi.org/10.1046/j.1365-2583.1998.72055.xOther links
http://hdl.handle.net/2299/16727Metadata
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