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dc.contributor.authorHoeller, Ulrich
dc.contributor.authorBaur, Manuela
dc.contributor.authorRoos, Franz F
dc.contributor.authorBrennan, Lorraine
dc.contributor.authorDaniel, Hannelore
dc.contributor.authorFallaize, Rosalind
dc.contributor.authorForster, Hannah
dc.contributor.authorGibney, Eileen R.
dc.contributor.authorGibney, Mike
dc.contributor.authorGodlewska, Magdalena
dc.contributor.authorHartwig, Kai
dc.contributor.authorKolossa, Silvia
dc.contributor.authorLambrinou, Christina P.
dc.contributor.authorLivingstone, Katherine M.
dc.contributor.authorLovegrove, Julie A.
dc.contributor.authorMacready, Anna L.
dc.contributor.authorManios, Yannis
dc.contributor.authorMarsaux, Cyril F M
dc.contributor.authorMartinez, J. Alfredo
dc.contributor.authorCelis-Morales, Carlos
dc.contributor.authorMoschonis, George
dc.contributor.authorNavas-Carretero, Santiago
dc.contributor.authorO'Donovan, Clare B.
dc.contributor.authorSan-Cristobal, Rodrigo
dc.contributor.authorSaris, Wim H. M.
dc.contributor.authorSurwiłło, Agnieszka
dc.contributor.authorTraczyk, Iwona
dc.contributor.authorTsirigoti, Lydia
dc.contributor.authorWalsh, Marianne C.
dc.contributor.authorWoolhead, Clara
dc.contributor.authorMathers, John C.
dc.contributor.authorWeber, Peter
dc.identifier.citationHoeller , U , Baur , M , Roos , F F , Brennan , L , Daniel , H , Fallaize , R , Forster , H , Gibney , E R , Gibney , M , Godlewska , M , Hartwig , K , Kolossa , S , Lambrinou , C P , Livingstone , K M , Lovegrove , J A , Macready , A L , Manios , Y , Marsaux , C F M , Martinez , J A , Celis-Morales , C , Moschonis , G , Navas-Carretero , S , O'Donovan , C B , San-Cristobal , R , Saris , W H M , Surwiłło , A , Traczyk , I , Tsirigoti , L , Walsh , M C , Woolhead , C , Mathers , J C & Weber , P 2016 , ' Application of dried blood spots to determine vitamin D status in a large nutritional study with unsupervised sampling : the Food4Me project ' , British Journal of Nutrition , vol. 115 , no. 2 , pp. 202-211 .
dc.identifier.otherPURE: 9476037
dc.identifier.otherPURE UUID: e0215d39-0962-419b-911d-3559cec057a9
dc.identifier.otherPubMed: 26548417
dc.identifier.otherScopus: 84951558509
dc.description.abstractAn efficient and robust method to measure vitamin D (25-hydroxy vitamin D3 (25(OH)D3) and 25-hydroxy vitamin D2 in dried blood spots (DBS) has been developed and applied in the pan-European multi-centre, internet-based, personalised nutrition intervention study Food4Me. The method includes calibration with blood containing endogenous 25(OH)D3, spotted as DBS and corrected for haematocrit content. The methodology was validated following international standards. The performance characteristics did not reach those of the current gold standard liquid chromatography-MS/MS in plasma for all parameters, but were found to be very suitable for status-level determination under field conditions. DBS sample quality was very high, and 3778 measurements of 25(OH)D3 were obtained from 1465 participants. The study centre and the season within the study centre were very good predictors of 25(OH)D3 levels (P<0·001 for each case). Seasonal effects were modelled by fitting a sine function with a minimum 25(OH)D3 level on 20 January and a maximum on 21 July. The seasonal amplitude varied from centre to centre. The largest difference between winter and summer levels was found in Germany and the smallest in Poland. The model was cross-validated to determine the consistency of the predictions and the performance of the DBS method. The Pearson's correlation between the measured values and the predicted values was r 0·65, and the sd of their differences was 21·2 nmol/l. This includes the analytical variation and the biological variation within subjects. Overall, DBS obtained by unsupervised sampling of the participants at home was a viable methodology for obtaining vitamin D status information in a large nutritional study.en
dc.relation.ispartofBritish Journal of Nutrition
dc.titleApplication of dried blood spots to determine vitamin D status in a large nutritional study with unsupervised sampling : the Food4Me projecten
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionWeight and Obesity Research Group
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionFood Policy, Nutrition and Diet
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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