dc.contributor.author | Uzoewulu, Gertrude | |
dc.contributor.author | Lawson, Lovett | |
dc.contributor.author | Nnanna, Ibeh | |
dc.contributor.author | Rastogi, Nalin | |
dc.contributor.author | Goyal, Madhu | |
dc.date.accessioned | 2016-04-27T11:09:35Z | |
dc.date.available | 2016-04-27T11:09:35Z | |
dc.date.issued | 2017-02-08 | |
dc.identifier.citation | Uzoewulu , G , Lawson , L , Nnanna , I , Rastogi , N & Goyal , M 2017 , ' Genetic Diversity of Mycobacterium tuberculosis Complex Strains Isolated from Patients with Pulmonary Tuberculosis in Anambra State, Nigeria ' , International Journal of Mycobacteriology , vol. 5 , no. 1 , pp. 74-79 . https://doi.org/10.1016/j.ijmyco.2015.06.008 | |
dc.identifier.issn | 2212-554X | |
dc.identifier.uri | http://hdl.handle.net/2299/17142 | |
dc.description | Open Access funded by Asian-African Society for Mycobacteriology. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike License CC BY-NC-SA 4.0 (https://creativecommons.org/licenses/by-nc-sa/4.0/ ) which permits others to remix, tweak, and build upon the work non-commercially, as long as they credit the author(s) and the publisher and license their new creations under the identical terms. | |
dc.description.abstract | In this study, we analyzed Mycobacterium tuberculosis complex (MTC) genetic diversity in Anambra State, Nigeria based on spoligotyping followed by 5-loci exact tandem repeats (ETRs). Spoligotyping of 180 MTC strains isolated in 2009–2011 from pulmonary tuberculosis (TB) patients led to a total of 31 distinct patterns. A comparison with the SITVIT2 international database showed that all the 31 patterns could be classified as Shared-types (SITs) in this database; briefly, 26/31 SITs (n = 174 isolates) matched a preexisting shared-type in the database, whereas 5/31 SITs (n = 6 isolates) were newly created due to 2 or more strains belonging to an identical new pattern within this study (SIT3396) or after a match with an orphan in the database (SIT3397, SIT3398, SIT3399 and SIT3400). A total of 18/31 SITs containing 167 or 92.8% isolates were clustered within this study (2–89 isolates per cluster) while 13/31 SITs contained unique strains. Using VNTR typing, a total of 36 distinct patterns were identified; 27 patterns (n = 157 isolates) matched a pattern already reported in the SITVIT2 database. Combination of both the methods generated 47 combined patterns for the 180 strains: 17 belonged to clustered isolates (n = 127 isolates or 70.5%) while 30 corresponded to as many unique strains (note 23 strains could not be typed using 5-loci ETRs). No correlation was found between the spoligotyping pattern and the HIV status of the patient or drug sensitivity of the strain. This study showed that the LAM10-CAM prototype SIT61 accounted for highest number of isolates (n = 89) in Anambra State, showing its relative contribution to the TB burden in the study. | en |
dc.format.extent | 6 | |
dc.format.extent | 887521 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Mycobacteriology | |
dc.subject | Mycobacterium tuberculosis, Spoligotyping | |
dc.title | Genetic Diversity of Mycobacterium tuberculosis Complex Strains Isolated from Patients with Pulmonary Tuberculosis in Anambra State, Nigeria | en |
dc.contributor.institution | Veterinary Science | |
dc.contributor.institution | Microbiology and Biotechnology | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Centre for Future Societies Research | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1016/j.ijmyco.2015.06.008 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |