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dc.contributor.authorUzoewulu, Gertrude
dc.contributor.authorLawson, Lovett
dc.contributor.authorNnanna, Ibeh
dc.contributor.authorRastogi, Nalin
dc.contributor.authorGoyal, Madhu
dc.date.accessioned2016-04-27T11:09:35Z
dc.date.available2016-04-27T11:09:35Z
dc.date.issued2017-02-08
dc.identifier.citationUzoewulu , G , Lawson , L , Nnanna , I , Rastogi , N & Goyal , M 2017 , ' Genetic Diversity of Mycobacterium tuberculosis Complex Strains Isolated from Patients with Pulmonary Tuberculosis in Anambra State, Nigeria ' , International Journal of Mycobacteriology , vol. 5 , no. 1 , pp. 74-79 . https://doi.org/10.1016/j.ijmyco.2015.06.008
dc.identifier.issn2212-554X
dc.identifier.otherPURE: 10002024
dc.identifier.otherPURE UUID: 7e1ac844-f626-485b-9775-98526c548490
dc.identifier.otherScopus: 84959142569
dc.identifier.urihttp://hdl.handle.net/2299/17142
dc.descriptionOpen Access funded by Asian-African Society for Mycobacteriology. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike License CC BY-NC-SA 4.0 (https://creativecommons.org/licenses/by-nc-sa/4.0/ ) which permits others to remix, tweak, and build upon the work non-commercially, as long as they credit the author(s) and the publisher and license their new creations under the identical terms.
dc.description.abstractIn this study, we analyzed Mycobacterium tuberculosis complex (MTC) genetic diversity in Anambra State, Nigeria based on spoligotyping followed by 5-loci exact tandem repeats (ETRs). Spoligotyping of 180 MTC strains isolated in 2009–2011 from pulmonary tuberculosis (TB) patients led to a total of 31 distinct patterns. A comparison with the SITVIT2 international database showed that all the 31 patterns could be classified as Shared-types (SITs) in this database; briefly, 26/31 SITs (n = 174 isolates) matched a preexisting shared-type in the database, whereas 5/31 SITs (n = 6 isolates) were newly created due to 2 or more strains belonging to an identical new pattern within this study (SIT3396) or after a match with an orphan in the database (SIT3397, SIT3398, SIT3399 and SIT3400). A total of 18/31 SITs containing 167 or 92.8% isolates were clustered within this study (2–89 isolates per cluster) while 13/31 SITs contained unique strains. Using VNTR typing, a total of 36 distinct patterns were identified; 27 patterns (n = 157 isolates) matched a pattern already reported in the SITVIT2 database. Combination of both the methods generated 47 combined patterns for the 180 strains: 17 belonged to clustered isolates (n = 127 isolates or 70.5%) while 30 corresponded to as many unique strains (note 23 strains could not be typed using 5-loci ETRs). No correlation was found between the spoligotyping pattern and the HIV status of the patient or drug sensitivity of the strain. This study showed that the LAM10-CAM prototype SIT61 accounted for highest number of isolates (n = 89) in Anambra State, showing its relative contribution to the TB burden in the study.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofInternational Journal of Mycobacteriology
dc.rights/dk/atira/pure/core/openaccesspermission/open
dc.subjectMycobacterium tuberculosis, Spoligotyping
dc.titleGenetic Diversity of Mycobacterium tuberculosis Complex Strains Isolated from Patients with Pulmonary Tuberculosis in Anambra State, Nigeriaen
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionVeterinary Science
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2015-06-11
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.ijmyco.2015.06.008
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeopenAccess


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