Show simple item record

dc.contributor.authorCrowther, Grace S.
dc.contributor.authorChilton, Caroline H.
dc.contributor.authorTodhunter, Sharie L.
dc.contributor.authorNicholson, Scott
dc.contributor.authorFreeman, Jane
dc.contributor.authorBaines, Simon D.
dc.contributor.authorWilcox, Mark H.
dc.date.accessioned2017-01-12T13:42:35Z
dc.date.available2017-01-12T13:42:35Z
dc.date.issued2014-04-30
dc.identifier.citationCrowther , G S , Chilton , C H , Todhunter , S L , Nicholson , S , Freeman , J , Baines , S D & Wilcox , M H 2014 , ' Comparison of planktonic and biofilm-associated communities of Clostridium difficile and indigenous gut microbiota in a triple-stage chemostat gut model ' , Journal of Antimicrobial Chemotherapy , vol. 69 , no. 8 , pp. 2137-2147 . https://doi.org/10.1093/jac/dku116
dc.identifier.issn0305-7453
dc.identifier.urihttp://hdl.handle.net/2299/17502
dc.descriptionGrace S. Crowther, et al, 'Comparison of planktonic and biofilm-associated communities of Clostridium difficile and indigenous gut microbiota in a triple-stage chemostat gut model', Journal of Antimicrobial Chemotherapy, April 2014, doi: 10.1093/jac/dku116 © The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
dc.description.abstractBACKGROUND: Biofilms are characteristic of some chronic or recurrent infections and this mode of growth tends to reduce treatment efficacy. Clostridium difficile infection (CDI) is associated with a high rate of recurrent symptomatic disease. The presence and behaviour of C. difficile within intestinal biofilms remains largely unexplored, but may factor in recurrent infection. METHODS: A triple-stage chemostat gut model designed to facilitate the formation of intestinal biofilm was inoculated with a pooled human faecal emulsion. Bacterial populations were allowed to equilibrate before simulated CDI was induced by clindamycin (33.9 mg/L, four times daily, 7 days) and subsequently treated with vancomycin (125 mg/L, four times daily, 7 days). Indigenous gut microbiota, C. difficile total viable counts, spores, cytotoxin and antimicrobial activity in planktonic and biofilm communities were monitored during the 10 week experimental period. RESULTS: Vancomycin successfully treated the initial episode of simulated CDI, but ∼18 days after therapy cessation, recurrent infection occurred. Germination, proliferation and toxin production were evident within planktonic communities in both initial and recurrent CDI. In contrast, sessile C. difficile remained in dormant spore form for the duration of the experiment. The effects of and recovery from clindamycin and vancomycin exposure for sessile populations was delayed compared with responses for planktonic bacteria. CONCLUSIONS: Intestinal biofilms provide a potential reservoir for C. difficile spore persistence, possibly facilitating their dispersal into the gut lumen after therapeutic intervention, leading to recurrent infection. Therapeutic options for CDI could have increased efficacy if they are more effective against sessile C. difficile.en
dc.format.extent527204
dc.language.isoeng
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
dc.subjectSESSILE
dc.subjectRECURRENCE
dc.subjectSPORES
dc.titleComparison of planktonic and biofilm-associated communities of Clostridium difficile and indigenous gut microbiota in a triple-stage chemostat gut modelen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionCentre for Research in Mechanisms of Disease and Drug Discovery
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Future Societies Research
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1093/jac/dku116
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record