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dc.contributor.authorChau, David
dc.contributor.authorDennis, Anthony
dc.contributor.authorLin, Hungyen
dc.contributor.authorZeitler, J Axel
dc.contributor.authorTunnacliffe, Alan
dc.date.accessioned2017-01-12T13:42:53Z
dc.date.available2017-01-12T13:42:53Z
dc.date.issued2016-02-01
dc.identifier.citationChau , D , Dennis , A , Lin , H , Zeitler , J A & Tunnacliffe , A 2016 , ' Determination of Water Content in Dehydrated Mammalian Cells Using Terahertz Pulsed Imaging: A Feasibility Study ' , Current Pharmaceutical Biotechnology , vol. 17 , no. 2 , pp. 200-207 . https://doi.org/10.2174/1389201017666151029105941
dc.identifier.issn1389-2010
dc.identifier.urihttp://hdl.handle.net/2299/17504
dc.descriptionThis is the accepted version of the following article: Chau, D., Dennis, A. R., Lin, H., Zeitler, J. A., Tunnacliffe, A., ‘Determination of Water Content in Dehydrated Mammalian Cells Using Terahertz Pulsed Imaging: A Feasibility Study’, Current Pharmaceutical Biotechnology 17(2): 200-207, February 2016. Subject to 12 months' embargo. Embargo end date: 1 February 2017. The published manuscript is available at http://www.eurekaselect.com/136239/article
dc.description.abstractMammalian cells are involved in a range of biotechnological applications and more recently have been increasingly exploited in regenerative medicine. Critical to successful applications involving mammalian cells are their long-term storage and transport, for which cryopreservation in liquid nitrogen is the most frequently used strategy. However, cryopreservation suffers from high costs, difficulties in transport logistics and the use of undesirable additives (e.g. animal sera or DMSO). An alternative approach, proposed as low cost, low maintenance and process-compatible, is viable desiccation of mammalian cells. Several groups claim to have achieved this, but the extent of desiccation in the cell samples concerned is not always clear, in part because of difficulties in determining very low water content. Although several techniques exist that are frequently used to quantify the amount of water in samples (e.g. FTIR spectroscopy, thermogravimetric analysis (TGA), NMR spectroscopy), the complexity of sample preparation, as well as the costs and time constraints involved are disadvantageous. Here, we assess a novel, rapid and low cost technique, i.e. terahertz (THz) spectroscopy, for the quantification of water content within dehydrated mammalian cell samples.en
dc.format.extent8
dc.format.extent1584383
dc.language.isoeng
dc.relation.ispartofCurrent Pharmaceutical Biotechnology
dc.subjectdehydration
dc.subjectdessication
dc.subjectterahertz pulsed imaging
dc.subjecthumidity
dc.subjectanhydrobiotic engineering
dc.titleDetermination of Water Content in Dehydrated Mammalian Cells Using Terahertz Pulsed Imaging: A Feasibility Studyen
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Pharmacy, Pharmacology and Postgraduate Medicine
dc.description.statusPeer reviewed
dc.date.embargoedUntil2017-02-01
rioxxterms.versionofrecord10.2174/1389201017666151029105941
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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