Show simple item record

dc.contributor.authorNullens, Sarah
dc.contributor.authorDeiteren, Annemie
dc.contributor.authorJiang, Wen
dc.contributor.authorKeating, Christopher
dc.contributor.authorCeuleers, Hannah
dc.contributor.authorFranque, Sven
dc.contributor.authorGrundy, David
dc.contributor.authorde Man, Joris de Man
dc.contributor.authorde Winter, Benedicte
dc.date.accessioned2017-02-06T17:29:16Z
dc.date.available2017-02-06T17:29:16Z
dc.date.issued2016-10-25
dc.identifier.citationNullens , S , Deiteren , A , Jiang , W , Keating , C , Ceuleers , H , Franque , S , Grundy , D , de Man , J D M & de Winter , B 2016 , ' In vitro recording of mesenteric afferent nerve activity in jejunal and colonic segments from rodents ' , Journal of Visualised Experiments , vol. 116 , e54576 . https://doi.org/10.3791/54576
dc.identifier.otherPURE: 9889979
dc.identifier.otherPURE UUID: 85d516c2-1b74-4d99-a466-331b327567f9
dc.identifier.otherScopus: 84992530849
dc.identifier.urihttp://hdl.handle.net/2299/17599
dc.descriptionThis is the accepted manuscript version of the following article: Nullens, S., Deiteren, A., Jiang, W., Keating, C., Ceuleers, H., Francque, S., Grundy, D., De Man, J.G., De Winter, B.Y, 'In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments', Journal of Visual Experiments (JOVE), e54576, October 2016, available online at doi:10.3791/54576 (2016). Copyright © 2016 Journal of Visualized Experiments The video component of this article can be found at http://www.jove.com/video/54576/
dc.description.abstractAfferent nerves not only convey information concerning normal physiology, but also signal disturbed homeostasis and pathophysiological processes of the different organ systems from the periphery towards the central nervous system. As such, the increased activity or 'sensitization' of mesenteric afferent nerves has been allocated an important role in the pathophysiology of visceral hypersensitivity and abdominal pain syndromes. Mesenteric afferent nerve activity can be measured in vitro in an isolated intestinal segment that is mounted in a purpose-built organ bath and from which the splanchnic nerve is isolated, allowing researchers to directly assess nerve activity adjacent to the gastrointestinal segment. Activity can be recorded at baseline in standardized conditions, during distension of the segment or following the addition of pharmacological compounds delivered intraluminally or serosally. This technique allows the researcher to easily study the effect of drugs targeting the peripheral nervous system in control specimens; besides, it provides crucial information on how neuronal activity is altered during disease. It should be noted however that measuring afferent neuronal firing activity only constitutes one relay station in the complex neuronal signaling cascade, and researchers should bear in mind not to overlook neuronal activity at other levels (e.g., dorsal root ganglia, spinal cord or central nervous system) in order to fully elucidate the complex neuronal physiology in health and disease. Commonly used applications include the study of neuronal activity in response to the administration of lipopolysaccharide, and the study of afferent nerve activity in animal models of irritable bowel syndrome. In a more translational approach, the isolated mouse intestinal segment can be exposed to colonic supernatants from IBS patients. Furthermore, a modification of this technique has been recently shown to be applicable in human colonic specimens.en
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofJournal of Visualised Experiments
dc.rightsOpen
dc.subjectneuroscience
dc.subjectvisceral hypersensitivity
dc.subjectpain
dc.subjectmesenteric nerve activity
dc.subjectjejunal afferent nerve activity
dc.subjectin vitro recording of afferent nerve activity
dc.subjectneuronal activity
dc.subjectmechanosensitivity
dc.subjectcolonic distension
dc.titleIn vitro recording of mesenteric afferent nerve activity in jejunal and colonic segments from rodentsen
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Pharmacy, Pharmacology and Postgraduate Medicine
dc.contributor.institutionBasic and Clinical Science Unit
dc.contributor.institutionCentre for Health Services and Clinical Research
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.jove.com/video/54576/in-vitro-recording-mesenteric-afferent-nerve-activity-mouse-jejunal
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Accepted Version
dcterms.dateAccepted2016-10-25
rioxxterms.versionAM
rioxxterms.versionofrecordhttps://doi.org/10.3791/54576
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record