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        Bioluminescent Francisella tularensis SCHU S4 enables non-invasive tracking of bacterial dissemination and the evaluation of antibiotics in an inhalational mouse model of tularaemia

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        AAC01586_16_2.pdf (PDF, 2Mb)
        Author
        Hall, Charlotte
        Flick-Smith, Helen
        Harding, Sarah
        Atkins, Helen
        Titball, Richard
        Attention
        2299/18345
        Abstract
        Bioluminescence imaging (BLI) enables real-time, noninvasive tracking of infection in vivo and longitudinal infection studies. In this study, a bioluminescent Francisella tularensis strain, SCHU S4-lux, was used to develop an inhalational infection model in BALB/c mice. Mice were infected intranasally, and the progression of infection was monitored in real time using BLI. A bioluminescent signal was detectable from 3 days postinfection (3 dpi), initially in the spleen and then in the liver and lymph nodes, before finally becoming systemic. The level of bioluminescent signal correlated with bacterial numbers in vivo, enabling noninvasive quantification of bacterial burdens in tissues. Treatment with levofloxacin (commencing at 4 dpi) significantly reduced the BLI signal. Furthermore, BLI was able to distinguish noninvasively between different levofloxacin treatment regimens and to identify sites of relapse following treatment cessation. These data demonstrate that BLI and SCHU S4-lux are suitable for the study of F. tularensis pathogenesis and the evaluation of therapeutics for tularemia.
        Publication date
        2016-12-01
        Published in
        Antimicrobial Agents and Chemotherapy
        Published version
        https://doi.org/10.1128/AAC.01586-16
        Other links
        http://hdl.handle.net/2299/18345
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