dc.contributor.author | Li, Wen | |
dc.contributor.author | Cheong, Yuen-Ki | |
dc.contributor.author | Wang, Hui | |
dc.contributor.author | Ren, Guogang | |
dc.contributor.author | Yang, Zhuo | |
dc.date.accessioned | 2017-08-17T15:55:31Z | |
dc.date.available | 2017-08-17T15:55:31Z | |
dc.date.issued | 2016-04-01 | |
dc.identifier.citation | Li , W , Cheong , Y-K , Wang , H , Ren , G & Yang , Z 2016 , ' Neuroprotective Effects of Etidronate and 2,3,3-Trisphosphonate Against Glutamate-Induced Toxicity in PC12 Cells ' , Neurochemical research , vol. 41 , no. 4 , pp. 844-854 . https://doi.org/10.1007/s11064-015-1761-4 | |
dc.identifier.issn | 0364-3190 | |
dc.identifier.other | ORCID: /0000-0001-8865-1526/work/62749472 | |
dc.identifier.uri | http://hdl.handle.net/2299/19234 | |
dc.description | This document is the Accepted Manuscript version of the following article: Wen Li, Yuen-Ki Cheong, Hui Wang, Guogang Ren and Zhuo Yang, ‘Neuroprotective Effects of Etidronate and 2,3,3-Trisphosphonate Against Glutamate-Induced Toxicity in PC12 Cells’, Neurochemical Research, Vol. 41 (4): 844-854, April 2016. The final publication is available at Springer via https://doi.org/10.1007/s11064-015-1761-4. | |
dc.description.abstract | Etidronate is one of the best known bisphosphonates (BP) derivatives. It is often used as a reference drug in research related to hypercalcaemia and other common bone diseases. 2,3,3-trisphosphonate (TrisPP) is brand new analogue of BP, that also contains a ‘germinal bisphosphonate’ unit with an additional phosphoryl group attached in proximity to the BP unit. It is known that BPs bind to calcium by chemisorptions to form Ca-BP complexes through (O)P–C–P(O) moiety and hydrogen coordinations, and so they suppress calcium flow by interfering with Ca2+ channel operations. The mechanistic actions of BP, involving interactions and regulations of Ca2+, are somewhat similar to the pathogenesis of well-known neurodegenerative disorders, such as Alzheimer’s disease, Parkinson’s disease and Huntington’s disease. To investigate if neuroprotective effects are exhibited by the compounds of interests, we used a rat adrenal pheochromocytoma cell line (PC12) as our in vitro model to observe any occurrence of neuron inter-reflection. We pre-treated these PC12 cells with etidronate and TrisPP before challenging the cells with a high concentration of the neurotoxin, glutamate. Our data showed that pre-treatment with 100 μM etidronate partially ameliorated the glutamate-induced decrease in cell viability (47 %), whereas pre-treating cells with 10–100 μM TrisPP showed remarkable cell protection (78–86 %). Moreover, pre-treatments of the cells with etidronate or TrisPP attenuated cell apoptosis, reactive oxygen species generation, Ca2+ overloading and caspase-3 protein expression, which were associated with a remarkable increase in superoxide dismutase activity in our glutamate-injured PC12 cells. Therefore, this study supports the notion that etidronate and TrisPP may be promising neuroprotective agents. | en |
dc.format.extent | 11 | |
dc.format.extent | 22978361 | |
dc.language.iso | eng | |
dc.relation.ispartof | Neurochemical research | |
dc.subject | 2,3,3-Trisphosphonate | |
dc.subject | Etidronate | |
dc.subject | Glutamate | |
dc.subject | Neuroprotection | |
dc.subject | PC12 cells | |
dc.subject | Cellular and Molecular Neuroscience | |
dc.subject | Biochemistry | |
dc.title | Neuroprotective Effects of Etidronate and 2,3,3-Trisphosphonate Against Glutamate-Induced Toxicity in PC12 Cells | en |
dc.contributor.institution | School of Engineering and Technology | |
dc.contributor.institution | Centre for Engineering Research | |
dc.contributor.institution | Materials and Structures | |
dc.description.status | Peer reviewed | |
dc.date.embargoedUntil | 2016-11-11 | |
rioxxterms.versionofrecord | 10.1007/s11064-015-1761-4 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |