dc.contributor.author | Bokori-Brown, Monika | |
dc.contributor.author | Hall, Charlotte | |
dc.contributor.author | Vance, Charlotte | |
dc.contributor.author | Fernandes da Costa, SP | |
dc.contributor.author | Savva, CG | |
dc.contributor.author | Naylor, Claire | |
dc.contributor.author | Cole, AR | |
dc.contributor.author | Basak, AK | |
dc.contributor.author | Moss, DS | |
dc.contributor.author | Titball, Richard | |
dc.date.accessioned | 2017-09-14T16:44:51Z | |
dc.date.available | 2017-09-14T16:44:51Z | |
dc.date.issued | 2014-05-13 | |
dc.identifier.citation | Bokori-Brown , M , Hall , C , Vance , C , Fernandes da Costa , SP , Savva , CG , Naylor , C , Cole , AR , Basak , AK , Moss , DS & Titball , R 2014 , ' Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia ' , Vaccine , vol. 32 , no. 23 , pp. 2682-2687 . https://doi.org/10.1016/j.vaccine.2014.03.079 | |
dc.identifier.issn | 0264-410X | |
dc.identifier.other | PURE: 10630918 | |
dc.identifier.other | PURE UUID: 1cf43435-73d1-4bb1-8001-b16ce7d1f093 | |
dc.identifier.other | Scopus: 84899977403 | |
dc.identifier.uri | http://hdl.handle.net/2299/19403 | |
dc.description | This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence ( https://creativecommons.org/licenses/by/3.0/ )which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2014 The Authors. Published by Elsevier Ltd. | |
dc.description.abstract | Epsilon toxin (Etx) is a β-pore-forming toxin produced by Clostridium perfringens toxinotypes B and D and plays a key role in the pathogenesis of enterotoxemia, a severe, often fatal disease of ruminants that causes significant economic losses to the farming industry worldwide. This study aimed to determine the potential of a site-directed mutant of Etx (Y30A-Y196A) to be exploited as a recombinant vaccine against enterotoxemia. Replacement of Y30 and Y196 with alanine generated a stable variant of Etx with significantly reduced cell binding and cytotoxic activities in MDCK.2 cells relative to wild type toxin (>430-fold increase in CT50) and Y30A-Y196A was inactive in mice after intraperitoneal administration of trypsin activated toxin at 1000× the expected LD50 dose of trypsin activated wild type toxin. Moreover, polyclonal antibody raised in rabbits against Y30A-Y196A provided protection against wild type toxin in an in vitro neutralisation assay. These data suggest that Y30A-Y196A mutant could form the basis of an improved recombinant vaccine against enterotoxemia. | en |
dc.format.extent | 6 | |
dc.language.iso | eng | |
dc.relation.ispartof | Vaccine | |
dc.title | Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Centre for Research into Topical Drug Delivery and Toxicology | |
dc.contributor.institution | Toxicology | |
dc.description.status | Peer reviewed | |
rioxxterms.version | VoR | |
rioxxterms.versionofrecord | https://doi.org/10.1016/j.vaccine.2014.03.079 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |