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dc.contributor.authorKarandeni Dewage, Chinthani Shanika
dc.contributor.authorWijerathna, Kavithra Jayani
dc.contributor.authorStotz, Henrik U.
dc.contributor.authorFitt, Bruce D. L.
dc.contributor.editorHull, Richard
dc.contributor.editorJellis, Graham
dc.contributor.editorMay, Mike
dc.contributor.editorMiller, Paul
dc.contributor.editorMoss, Stephen
dc.contributor.editorNicholls, Caroline
dc.contributor.editorOrson, Jim
dc.date.accessioned2017-09-27T10:56:38Z
dc.date.available2017-09-27T10:56:38Z
dc.date.issued2017-02-28
dc.identifier.citationKarandeni Dewage , C S , Wijerathna , K J , Stotz , H U & Fitt , B D L 2017 , Improved understanding of novel sources of resistance against the light leaf spot pathogen, Pyrenopeziza brassicae . in R Hull , G Jellis , M May , P Miller , S Moss , C Nicholls & J Orson (eds) , Crop Production in Southern Britain 2017 . vol. 134 , 134 , The Association of Applied Biologists , Warwick , pp. 221-226 , Crop Production in Southern Britain , Peterborough , United Kingdom , 15/02/17 . < http://www.aab.org.uk/contentok.php?id=501 >
dc.identifier.citationconference
dc.identifier.otherPURE: 11358840
dc.identifier.otherPURE UUID: 57cb9841-3cee-43e4-9fe8-c1d06c7a305b
dc.identifier.urihttp://hdl.handle.net/2299/19449
dc.descriptionChinthani Shanika Karandeni Dewage, Kavithra Jayani Wijerathna, Henrik U. Stotz, and Bruce D. L. Fitt, 'Improved understanding of novel sources of resistance against the light leaf spot pathogen, Pyrenopeziza brassicae', paper presented at the Association of Applied Biologists Conference Crop Production in Southern Britain 2017, 15 - 16 February 2017, Peterborough, UK. Proceedings available online at: http://www.aab.org.uk/contentok.php?id=501.
dc.description.abstractIn this work, the endophytic growth phase of the light leaf spot pathogen Pyrenopeziza brassicae in selected lines from a doubled haploid (DH) population of oilseed rape, which is known to segregate for resistance against P. brassicae, was characterised using controlled environment (CE) experiments. Fungal staining techniques and pathogen-specific quantitative polymerase chain reactions (qPCR) were used to observe and quantify the pathogen biomass, respectively. The qPCR results showed that the resistant lines contained little P. brassicae DNA and there seemed to be little to no change in the amount of DNA over time. In contrast, there was a considerable increase in pathogen DNA in susceptible lines from 0 to 24 days post inoculation (dpi). These results were also reflected in observations made by a fungal staining method. In addition, leaf samples of these DH lines, collected at three different times from winter oilseed rape field experiments, were analysed using qPCR. The resistant lines had a considerably smaller amount of P. brassicae DNA in leaf samples collected later in the cropping season than that in susceptible lines.en
dc.format.extent6
dc.language.isoeng
dc.publisherThe Association of Applied Biologists
dc.relation.ispartofCrop Production in Southern Britain 2017
dc.subjectoilseed rape
dc.subjectPyrenopeziza brassicae
dc.subjectyield loss
dc.subjectlight leaf spot
dc.subjecthost resistance
dc.subjectextracellular pathogens
dc.titleImproved understanding of novel sources of resistance against the light leaf spot pathogen, Pyrenopeziza brassicaeen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionWeight and Obesity Research Group
dc.identifier.urlhttp://www.aab.org.uk/contentok.php?id=501
rioxxterms.versionVoR
rioxxterms.typeOther
herts.preservation.rarelyaccessedtrue


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