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dc.contributor.authorStratton, Dan
dc.contributor.authorMoore, Colin
dc.contributor.authorZheng, Lei
dc.contributor.authorLange, Sigrun
dc.contributor.authorInal, Jameel
dc.identifier.citationStratton , D , Moore , C , Zheng , L , Lange , S & Inal , J 2015 , ' Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles ' , Biochemical and Biophysical Research Communications , vol. 460 , no. 3 , pp. 511-517 .
dc.identifier.otherPURE: 13068203
dc.identifier.otherPURE UUID: 5df486bf-1a0a-4631-b933-8b32263bd1c6
dc.identifier.otherScopus: 84937761183
dc.identifier.otherPubMed: 25797625
dc.description.abstractMVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, cells take at least 20 min to fully recover their ability to microvesiculate. This release of MVs through activation of sublytic C5b-9 was inhibited by the PKC inhibitor bisindoylmaleimide I but not the Rho kinase inhibitor, Y27632. After stimulus there is a rise of 79 nMs-1 over 11 s, reaching a peak [Ca2+]i of 920 nM. The concentration of cytosolic calcium then falls steadily at 2.4 nMs-1 over 109 s reaching baseline levels (50-100 nM) within 10-15 min. In PC3 cells the rate of release of MVs from stimulated cells also reaches a minimum within 10-15 min. Using fura-2 AM-loaded cells, upon stimulation, cells were found to release MVs with a concentration of intravesicular calcium estimated at ∼430 nM.en
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.subjectIntravesicular calcium
dc.subjectRefractory period
dc.subjectSublytic C5b-9
dc.subjectMolecular Biology
dc.subjectCell Biology
dc.titleProstate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesiclesen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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