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dc.contributor.authorStratton, Dan
dc.contributor.authorLange, Sigrun
dc.contributor.authorKholia, Sharad
dc.contributor.authorJorfi, Samireh
dc.contributor.authorAntwi-Baffour, Samuel
dc.contributor.authorInal, Jameel
dc.identifier.citationStratton , D , Lange , S , Kholia , S , Jorfi , S , Antwi-Baffour , S & Inal , J 2014 , ' Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance ' , Biochemical and Biophysical Research Communications , vol. 453 , no. 3 , pp. 619-624 .
dc.identifier.otherPURE: 13068971
dc.identifier.otherPURE UUID: 7418f012-0710-460b-b7eb-a30caa11a0b2
dc.identifier.otherScopus: 84918500340
dc.identifier.otherPubMed: 25301562
dc.description.abstractUsing a Quartz Crystal Microbalance with dissipation monitoring, QCM-D (label-free system) measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, we showed the attachment, over a 60 min period, of a monolayer of PC3 cells to the gold electrodes of the quartz crystal sensor, which had been rendered hydrophilic. That MVs were released upon BzATP stimulation of cells was confirmed by NTA analysis (average 250 nm diameter), flow cytometry, showing high phosphatidylserine exposition and by fluorescent (Annexin V Alexa Fluor® 488-positive) and electron microscopy. Over a period of 1000s (16.7 min) during which early apoptosis increased from 4% plateauing at 10% and late apoptosis rose to 2%, the Δf increased 20 Hz, thereupon remaining constant for the last 1000s of the experiment. Using the Sauerbrey equation, the loss in mass, which corresponded to the release of 2.36 × 106 MVs, was calculated to be 23 ng. We therefore estimated the mass of an MV to be 0.24 pg. With the deposition on the QCM-D of 3.5 × 107 MVs over 200s, the decrease in Δf (Hz) gave an estimate of 0.235 pg per MV.en
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.subjectProstate cancer cells (PC3)
dc.subjectQuartz Crystal Microbalance
dc.subjectReal-time analysis
dc.subjectStimulated release
dc.subjectMolecular Biology
dc.subjectCell Biology
dc.titleLabel-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalanceen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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