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dc.contributor.authorPatidar, Ashish
dc.contributor.authorSingh, Dhruv
dc.contributor.authorThakur, Shori
dc.contributor.authorWinocour, Peter
dc.contributor.authorFarrington, Kenneth
dc.contributor.authorBaydoun, Anwar
dc.date.accessioned2018-01-30T22:21:33Z
dc.date.available2018-01-30T22:21:33Z
dc.date.issued2017-05-09
dc.identifier.citationPatidar , A , Singh , D , Thakur , S , Winocour , P , Farrington , K & Baydoun , A 2017 , ' Diabetes confers in vitro calcific potential on serum which associates with in-vivo vascular calcification ' , Clinical Science , vol. 131 , no. 10 , CS20160882 , pp. 991-1000 . https://doi.org/10.1042/CS20160882
dc.identifier.issn0143-5221
dc.identifier.otherPURE: 11427892
dc.identifier.otherPURE UUID: ea494940-f302-4902-a03c-eabd3cca602e
dc.identifier.otherScopus: 85026289978
dc.identifier.urihttp://hdl.handle.net/2299/19650
dc.descriptionThis is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC-BY).
dc.description.abstractAlthough vascular calcification (VC) is prevalent in Type 2 diabetes mellitus (T2DM), underlying mechanisms remain unclear. Neither is it known whether T2DM confers calcific potential (CP) on serum, enabling it to induce VC outside the disease milieu. We, therefore, investigated the CP of serum from controls and subjects with T2DM with and without in vivo VC. Samples from 20 healthy controls and 44 age- and sex-matched patients with T2DM with modification of diet in renal disease estimated glomerular filtration rate (MDRD-4 eGFR) > 60 ml·min-1 were analysed for CP using rat aortic smooth muscle cells in vitro. CT scans of femoral arteries identified individuals with in vivo calcification. Serum from subjects with T2DM revealed significantly greater CP than controls. This was further enhanced in the presence of in vivo VC. Addition of β-glycerophosphate (β-GP) plus CaCl2 increased the CP of T2DM serum but not of controls. Along with age, CP was an independent predictor of the presence of VC. In receiver operator curve (ROC) analysis, CP was a significant predictor of femoral arterial VC (C-statistic 0.70: P=0.009). The distribution of CP was bimodal around a cutoff of 100 nmoles of Ca2+ protein mg-1, with a higher proportion of Type 2 diabetes subjects with in vivo calcification (T2DM+) sera above the cutoff value. This group also showed elevated levels of osteoprotegerin (OPG) and matrix Gla protein (MGP). Diabetes confers CP on the serum which is enhanced by the presence of in vivo VC. The CP acquired may be dependent on levels of OPG and MGP. These findings may be clinically relevant for early identification of individuals at risk of VC and for informing therapeutic strategies.en
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofClinical Science
dc.rightsOpen
dc.subjectSerum Calcific Potential
dc.subjectType 2 Diabetes
dc.titleDiabetes confers in vitro calcific potential on serum which associates with in-vivo vascular calcificationen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Pharmacy, Pharmacology and Postgraduate Medicine
dc.contributor.institutionBasic and Clinical Science Unit
dc.contributor.institutionCentre for Health Services and Clinical Research
dc.contributor.institutionPharmacology and Clinical Science Research
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2017-05-09
rioxxterms.versionAM
rioxxterms.versionP
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1042/CS20160882
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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