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dc.contributor.authorHoffman, Ewelina
dc.contributor.authorBodhaniya, Aateka
dc.contributor.authorBall, Doug
dc.contributor.authorKlapwijk, Jan
dc.contributor.authorMillar, Val
dc.contributor.authorKumar, Abhinav
dc.contributor.authorMartin, Abigail
dc.contributor.authorMahendran, Rhamiya
dc.contributor.authorDailey, Lea Ann
dc.contributor.authorForbes, Ben
dc.contributor.authorHutter, Victoria
dc.date.accessioned2018-01-30T22:24:06Z
dc.date.available2018-01-30T22:24:06Z
dc.date.issued2017-12-31
dc.identifier.citationHoffman , E , Bodhaniya , A , Ball , D , Klapwijk , J , Millar , V , Kumar , A , Martin , A , Mahendran , R , Dailey , L A , Forbes , B & Hutter , V 2017 , ' Morphometric Characterization of Rat and Human Alveolar Macrophage Cell Models and their Response to Amiodarone using High Content Image Analysis ' , Pharmaceutical Research , vol. 34 , no. 12 , pp. 2466-2476 . https://doi.org/10.1007/s11095-017-2176-5
dc.identifier.issn0724-8741
dc.identifier.otherPURE: 11875575
dc.identifier.otherPURE UUID: 16b03437-29a3-425b-aa24-2b6e4f06fad8
dc.identifier.otherScopus: 85019645576
dc.identifier.otherORCID: /0000-0002-7998-924X/work/62750777
dc.identifier.otherORCID: /0000-0002-4172-0827/work/68244650
dc.identifier.urihttp://hdl.handle.net/2299/19653
dc.description© The Author(s) 2017. This article is an open access publication. Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.description.abstractPurpose. Progress to the clinic may be delayed or prevented when vacuolated or “foamy” alveolar macrophages are observed during non-clinical inhalation toxicology assessment. The first step in developing methods to study this response in vitro is to characterize macrophage cell lines and their response to drug exposures.Methods. Human (U937) and rat (NR8383) cell lines and primary rat alveolar macrophages obtained by bronchoalveolar lavage were characterized using high content fluorescence imaging analysis quantification of cell viability, morphometry, and phospholipid and neutral lipid accumulation. Results. Cell health, morphology and lipid content were comparable (p<0.05) for both cell lines and the primary macrophages in terms of vacuole number, size and lipid content. Responses to amiodarone, a known inducer of phospholipidosis, required analysis of shifts in cell population profiles (the proportion of cells with elevated vacuolation or lipid content) rather than average population data which was insensitive to the changes observed.Conclusions. A high content image analysis assay was developed and used to provide detailed morphological characterization of rat and human alveolar-like macrophages and their response to a phospholipidosis-inducing agent. This provides a basis for development of assays to predict or understand macrophage vacuolation following inhaled drug exposure.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofPharmaceutical Research
dc.rightsOpen
dc.subjectfoamy macrophage
dc.subjectNR8383
dc.subjectU937
dc.subjecttoxicology
dc.subjectvacuolation
dc.subjectPharmacology, Toxicology and Pharmaceutics(all)
dc.titleMorphometric Characterization of Rat and Human Alveolar Macrophage Cell Models and their Response to Amiodarone using High Content Image Analysisen
dc.contributor.institutionDepartment of Pharmacy, Pharmacology and Postgraduate Medicine
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Clinical and Pharmaceutical Sciences
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionAirway Group
dc.contributor.institutionPharmaceutics
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2017-12-31
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1007/s11095-017-2176-5
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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