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dc.contributor.authorGonzález-Calle, Virginia
dc.contributor.authorBarrero-Sicilia, Cristina
dc.contributor.authorCarbonero, Pilar
dc.contributor.authorIglesias-Fernández, Raquel
dc.date.accessioned2018-01-30T22:24:15Z
dc.date.available2018-01-30T22:24:15Z
dc.date.issued2015-07-01
dc.identifier.citationGonzález-Calle , V , Barrero-Sicilia , C , Carbonero , P & Iglesias-Fernández , R 2015 , ' Mannans and endo-β-mannanases (MAN) in Brachypodium distachyon : Expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination ' , Journal of Experimental Botany , vol. 66 , no. 13 , pp. 3753-3764 . https://doi.org/10.1093/jxb/erv168
dc.identifier.issn0022-0957
dc.identifier.otherPURE: 11883792
dc.identifier.otherPURE UUID: 64283ac4-217a-4d6e-9eb7-d3990b75ff2f
dc.identifier.otherScopus: 84938277825
dc.identifier.otherPubMed: 25922488
dc.identifier.urihttp://hdl.handle.net/2299/19654
dc.description© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.description.abstractImmunolocalization of mannans in the seeds of Brachypodium distachyon reveals the presence of these polysaccharides in the root embryo and in the coleorhiza in the early stages of germination (12h), decreasing thereafter to the point of being hardly detected at 27h. Concurrently, the activity of endo-β-mannanases (MANs; EC 3.2.1.78) that catalyse the hydrolysis of β-1,4 bonds in mannan polymers, increases as germination progresses. The MAN gene family is represented by six members in the Brachypodium genome, and their expression has been explored in different organs and especially in germinating seeds. Transcripts of BdMAN2, BdMAN4 and BdMAN6 accumulate in embryos, with a maximum at 24-30h, and are detected in the coleorhiza and in the root by in situ hybridization analyses, before root protrusion (germination sensu stricto). BdMAN4 is not only present in the embryo root and coleorhiza, but is abundant in the de-embryonated (endosperm) imbibed seeds, while BdMAN2 and BdMAN6 are faintly expressed in endosperm during post-germination (36-42h). BdMAN4 and BdMAN6 transcripts are detected in the aleurone layer. These data indicate that BdMAN2, BdMAN4 and BdMAN6 are important for germination sensu stricto and that BdMAN4 and BdMAN6 may also influence reserve mobilization. Whether the coleorhiza in monocots and the micropylar endosperm in eudicots have similar functions, is discussed.en
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofJournal of Experimental Botany
dc.rightsOpen
dc.subjectBdMAN gene family
dc.subjectBrachypodium distachyon
dc.subjectcoleorhiza
dc.subjectendo-β-mannanases
dc.subjectgermination
dc.subjectMAN gene expression
dc.subjectmannan immunolocalization
dc.subjectmRNA in situ hybridization
dc.subjectPhysiology
dc.subjectPlant Science
dc.titleMannans and endo-β-mannanases (MAN) in Brachypodium distachyon : Expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germinationen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84938277825&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2015-07-01
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1093/jxb/erv168
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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