dc.contributor.author | Iglesias-Fernández, Raquel | |
dc.contributor.author | Wozny, Dorothee | |
dc.contributor.author | Iriondo-De Hond, Maite | |
dc.contributor.author | Oñate-Sánchez, Luis | |
dc.contributor.author | Carbonero, Pilar | |
dc.contributor.author | Barrero-Sicilia, Cristina | |
dc.date.accessioned | 2018-01-30T22:24:40Z | |
dc.date.available | 2018-01-30T22:24:40Z | |
dc.date.issued | 2014-05-01 | |
dc.identifier.citation | Iglesias-Fernández , R , Wozny , D , Iriondo-De Hond , M , Oñate-Sánchez , L , Carbonero , P & Barrero-Sicilia , C 2014 , ' The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1 ' , Journal of Experimental Botany , vol. 65 , no. 8 , pp. 2009-2021 . https://doi.org/10.1093/jxb/eru055 | |
dc.identifier.issn | 0022-0957 | |
dc.identifier.other | ORCID: /0000-0001-7544-5619/work/33811134 | |
dc.identifier.uri | http://hdl.handle.net/2299/19657 | |
dc.description | © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/ ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.description.abstract | Protein hydrolysis plays an important role during seed germination and post-germination seedling establishment. In Arabidopsis thaliana, cathepsin B-like proteases are encoded by a gene family of three members, but only the AtCathB3 gene is highly induced upon seed germination and at the early post-germination stage. Seeds of a homozygous T-DNA insertion mutant in the AtCathB3 gene have, besides a reduced cathepsin B activity, a slower germination than the wild type. To explore the transcriptional regulation of this gene, we used a combined phylogenetic shadowing approach together with a yeast one-hybrid screening of an arrayed library of approximately 1200 transcription factor open reading frames from Arabidopsis thaliana. We identified a conserved CathB3-element in the promoters of orthologous CathB3 genes within the Brassicaceae species analysed, and, as its DNA-interacting protein, the G-Box Binding Factor1 (GBF1). Transient overexpression of GBF1 together with a PAtCathB3::uidA (β-glucuronidase) construct in tobacco plants revealed a negative effect of GBF1 on expression driven by the AtCathB3 promoter. In stable P35S::GBF1 lines, not only was the expression of the AtCathB3 gene drastically reduced, but a significant slower germination was also observed. In the homozygous knockout mutant for the GBF1 gene, the opposite effect was found. These data indicate that GBF1 is a transcriptional repressor of the AtCathB3 gene and affects the germination kinetics of Arabidopsis thaliana seeds. As AtCathB3 is also expressed during post-germination in the cotyledons, a role for the AtCathB3-like protease in reserve mobilization is also inferred. | en |
dc.format.extent | 13 | |
dc.format.extent | 5802766 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Experimental Botany | |
dc.subject | Arabidopsis thaliana | |
dc.subject | AtCathB3 | |
dc.subject | bZIP | |
dc.subject | cathepsin B-like enzymes | |
dc.subject | GBF1 | |
dc.subject | phylogenetic shadowing | |
dc.subject | reserve mobilization | |
dc.subject | seed germination | |
dc.subject | transcriptional regulation | |
dc.subject | Physiology | |
dc.subject | Plant Science | |
dc.title | The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1 | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Centre for Future Societies Research | |
dc.contributor.institution | Centre for Agriculture, Food and Environmental Management Research | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=84899009600&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1093/jxb/eru055 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |