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dc.contributor.authorIglesias-Fernández, Raquel
dc.contributor.authorWozny, Dorothee
dc.contributor.authorIriondo-De Hond, Maite
dc.contributor.authorOñate-Sánchez, Luis
dc.contributor.authorCarbonero, Pilar
dc.contributor.authorBarrero-Sicilia, Cristina
dc.date.accessioned2018-01-30T22:24:40Z
dc.date.available2018-01-30T22:24:40Z
dc.date.issued2014-05-01
dc.identifier.citationIglesias-Fernández , R , Wozny , D , Iriondo-De Hond , M , Oñate-Sánchez , L , Carbonero , P & Barrero-Sicilia , C 2014 , ' The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1 ' , Journal of Experimental Botany , vol. 65 , no. 8 , pp. 2009-2021 . https://doi.org/10.1093/jxb/eru055
dc.identifier.issn0022-0957
dc.identifier.otherPURE: 11884570
dc.identifier.otherPURE UUID: 6a95f633-8d71-4289-bd15-07e2e0ba2f54
dc.identifier.otherScopus: 84899009600
dc.identifier.otherPubMed: 24600022
dc.identifier.otherORCID: /0000-0001-7544-5619/work/33811134
dc.identifier.urihttp://hdl.handle.net/2299/19657
dc.description© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/ ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.description.abstractProtein hydrolysis plays an important role during seed germination and post-germination seedling establishment. In Arabidopsis thaliana, cathepsin B-like proteases are encoded by a gene family of three members, but only the AtCathB3 gene is highly induced upon seed germination and at the early post-germination stage. Seeds of a homozygous T-DNA insertion mutant in the AtCathB3 gene have, besides a reduced cathepsin B activity, a slower germination than the wild type. To explore the transcriptional regulation of this gene, we used a combined phylogenetic shadowing approach together with a yeast one-hybrid screening of an arrayed library of approximately 1200 transcription factor open reading frames from Arabidopsis thaliana. We identified a conserved CathB3-element in the promoters of orthologous CathB3 genes within the Brassicaceae species analysed, and, as its DNA-interacting protein, the G-Box Binding Factor1 (GBF1). Transient overexpression of GBF1 together with a PAtCathB3::uidA (β-glucuronidase) construct in tobacco plants revealed a negative effect of GBF1 on expression driven by the AtCathB3 promoter. In stable P35S::GBF1 lines, not only was the expression of the AtCathB3 gene drastically reduced, but a significant slower germination was also observed. In the homozygous knockout mutant for the GBF1 gene, the opposite effect was found. These data indicate that GBF1 is a transcriptional repressor of the AtCathB3 gene and affects the germination kinetics of Arabidopsis thaliana seeds. As AtCathB3 is also expressed during post-germination in the cotyledons, a role for the AtCathB3-like protease in reserve mobilization is also inferred.en
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofJournal of Experimental Botany
dc.subjectArabidopsis thaliana
dc.subjectAtCathB3
dc.subjectbZIP
dc.subjectcathepsin B-like enzymes
dc.subjectGBF1
dc.subjectphylogenetic shadowing
dc.subjectreserve mobilization
dc.subjectseed germination
dc.subjecttranscriptional regulation
dc.subjectPhysiology
dc.subjectPlant Science
dc.titleThe AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1en
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84899009600&partnerID=8YFLogxK
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1093/jxb/eru055
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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