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dc.contributor.authorÖzkan, Selin
dc.contributor.authorMohorianu, Irina
dc.contributor.authorXu, Ping
dc.contributor.authorDalmay, Tamas
dc.contributor.authorCoutts, Robert H.A.
dc.date.accessioned2018-01-30T22:25:31Z
dc.date.available2018-01-30T22:25:31Z
dc.date.issued2017-05-30
dc.identifier.citationÖzkan , S , Mohorianu , I , Xu , P , Dalmay , T & Coutts , R H A 2017 , ' Profile and functional analysis of small RNAs derived from Aspergillus fumigatus infected with double-stranded RNA mycoviruses ' , BMC Genomics , vol. 18 , no. 1 , 416 . https://doi.org/10.1186/s12864-017-3773-8
dc.identifier.issn1471-2164
dc.identifier.otherPURE: 12010004
dc.identifier.otherPURE UUID: 4dea9823-f7df-44db-b48b-a95f48785eef
dc.identifier.otherScopus: 85019994567
dc.identifier.urihttp://hdl.handle.net/2299/19658
dc.description© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.description.abstractBackground: Mycoviruses are viruses that naturally infect and replicate in fungi. Aspergillus fumigatus, an opportunistic pathogen causing fungal lung diseases in humans and animals, was recently shown to harbour several different types of mycoviruses. A well-characterised defence against virus infection is RNA silencing. The A. fumigatus genome encodes essential components of the RNA silencing machinery, including Dicer, Argonaute and RNA-dependent RNA polymerase (RdRP) homologues. Active silencing of double-stranded (ds)RNA and the generation of small RNAs (sRNAs) has been shown for several mycoviruses and it is anticipated that a similar mechanism will be activated in A. fumigatus isolates infected with mycoviruses. Results: To investigate the existence and nature of A. fumigatus sRNAs, sRNA-seq libraries of virus-free and virus-infected isolates were created using Scriptminer adapters and compared. Three dsRNA viruses were investigated: Aspergillus fumigatus partitivirus-1 (AfuPV-1, PV), Aspergillus fumigatus chrysovirus (AfuCV, CV) and Aspergillus fumigatus tetramycovirus-1 (AfuTmV-1, NK) which were selected because they induce phenotypic changes such as coloration and sectoring. The dsRNAs of all three viruses, which included two conventionally encapsidated ones PV and CV and one unencapsidated example NK, were silenced and yielded characteristic vsiRNAs together with co-incidental silencing of host fungal genes which shared sequence homology with the viral genomes. Conclusions: Virus-derived sRNAs were detected and characterised in the presence of virus infection. Differentially expressed A. fumigatus microRNA-like (miRNA-like) sRNAs and small interfering RNAs (siRNAs) were detected and validated. Host sRNA loci which were differentially expressed as a result of virus infection were also identified. To our knowledge, this is the first study reporting the sRNA profiles of A. fumigatus isolates.en
dc.language.isoeng
dc.relation.ispartofBMC Genomics
dc.rightsOpen
dc.subjectAspergillus fumigatus
dc.subjectDifferential expression
dc.subjectDouble-stranded RNA mycovirus
dc.subjectFungal sRNA
dc.subjectMiRNA-like sRNA
dc.subjectSRNA-seq
dc.subjectVsiRNA
dc.subjectBiotechnology
dc.subjectGenetics
dc.titleProfile and functional analysis of small RNAs derived from Aspergillus fumigatus infected with double-stranded RNA mycovirusesen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionUniversity of Hertfordshire
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85019994567&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2017-05-30
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1186/s12864-017-3773-8
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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