dc.contributor.author | Kosgodage, Uchini S | |
dc.contributor.author | Trindade, Rita P | |
dc.contributor.author | Thompson, Paul R | |
dc.contributor.author | Inal, Jameel M | |
dc.contributor.author | Lange, Sigrun | |
dc.date.accessioned | 2018-01-30T22:31:51Z | |
dc.date.available | 2018-01-30T22:31:51Z | |
dc.date.issued | 2017-05-09 | |
dc.identifier.citation | Kosgodage , U S , Trindade , R P , Thompson , P R , Inal , J M & Lange , S 2017 , ' Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy ' , International Journal of Molecular Sciences (IJMS) , vol. 18 , no. 5 , 1007 . https://doi.org/10.3390/ijms18051007 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.other | PubMedCentral: PMC5454920 | |
dc.identifier.uri | http://hdl.handle.net/2299/19672 | |
dc.description | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0). | |
dc.description.abstract | Microvesicle (MV) release from tumour cells influences drug retention, contributing to cancer drug resistance. Strategically regulating MV release may increase drug retention within cancer cells and allow for lower doses of chemotherapeutic drugs. The contribution of exosomes to drug retention still remains unknown. Potential exosome and MV (EMV) biogenesis inhibitors, tested on human prostate cancer (PC3) cells for their capacity to inhibit EMV release, were also tested on PC3 and MCF-7 (breast cancer) cells for improving chemotherapy. Agents inhibiting EMV release most significantly, whilst maintaining cell viability, were chloramidine (Cl-amidine; 50 µM) and bisindolylmaleimide-I (10 µM). Apoptosis mediated by the chemotherapy drug 5-fluorouracil (5-FU) was significantly enhanced in PC3 cells in the presence of both these EMV inhibitors, resulting in a 62% (Cl-amidine + 5-FU) and 59% (bisindolylmaleimide-I + 5-FU) decrease in numbers of viable PC3 cells compared to 5-FU alone after 24 h. For MCF-7 cells, there were similar increased reductions of viable cells compared to 5-FU treatment alone ranging from 67% (Cl-amidine + 5-FU) to 58% (bisindolylmaleimide-I + 5-FU). Using combinatory treatment, the two EMV inhibitors further reduced the number of viable cancer cells tested. Neither inhibitor affected cell viability. Combining selected EMV inhibitors may pose as a novel strategy to enhance the efficacy of chemotherapeutic drug-mediated apoptosis. | en |
dc.format.extent | 12 | |
dc.format.extent | 1376648 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Molecular Sciences (IJMS) | |
dc.subject | Journal Article | |
dc.title | Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Biological and Environmental Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.3390/ijms18051007 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |