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dc.contributor.authorGarcia-Cano, Elena
dc.contributor.authorShimpei, Magori
dc.contributor.authorSun, Q
dc.contributor.authorDing, Z
dc.contributor.authorLazarowitz, SG
dc.contributor.authorCitovsky, Vitaly
dc.date.accessioned2018-01-30T22:33:47Z
dc.date.available2018-01-30T22:33:47Z
dc.date.issued2015-11-16
dc.identifier.citationGarcia-Cano , E , Shimpei , M , Sun , Q , Ding , Z , Lazarowitz , SG & Citovsky , V 2015 , ' Interaction of Arabidopsis Trihelix-Domain Transcription Factors VFP3 and VFP5 with Agrobacterium Virulence Protein VirF. ' , PLoS ONE . https://doi.org/10.1371/journal.pone.0142128
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 13227414
dc.identifier.otherPURE UUID: 1f99556d-12dd-4579-ad5d-a6d66aaf8811
dc.identifier.otherScopus: 84957112241
dc.identifier.urihttp://hdl.handle.net/2299/19685
dc.descriptionThis is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
dc.description.abstractAgrobacterium is a natural genetic engineer of plants that exports several virulence proteins into host cells in order to take advantage of the cell machinery to facilitate transformation and support bacterial growth. One of these effectors is the F-box protein VirF, which pre- sumably uses the host ubiquitin/proteasome system (UPS) to uncoat the packaging pro- teins from the invading bacterial T-DNA. By analogy to several other bacterial effectors, VirF most likely has several functions in the host cell and, therefore, several interacting part- ners among host proteins. Here we identify one such interactor, an Arabidopsis trihelix- domain transcription factor VFP3, and further show that its very close homolog VFP5 also interacted with VirF. Interestingly, interactions of VirF with either VFP3 or VFP5 did not acti- vate the host UPS, suggesting that VirF might play other UPS-independent roles in bacterial infection. To better understand the potential scope of VFP3 function, we used RNAi to reduce expression of the VFP3 gene. Transcriptome profiling of these VFP3-silenced plants using high-throughput cDNA sequencing (RNA-seq) revealed that VFP3 substantially affected plant gene expression; specifically, 1,118 genes representing approximately 5% of all expressed genes were significantly either up- or down-regulated in the VFP3 RNAi line compared to wild-type Col-0 plants. Among the 507 up-regulated genes were genes impli- cated in the regulation of transcription, protein degradation, calcium signaling, and hormone metabolism, whereas the 611 down-regulated genes included those involved in redox regu- lation, light reactions of photosynthesis, and metabolism of lipids, amino acids, and cell wall. Overall, this pattern of changes in gene expression is characteristic of plants under stress. Thus, VFP3 likely plays an important role in controlling plant homeostasis.en
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.rightsOpen
dc.titleInteraction of Arabidopsis Trihelix-Domain Transcription Factors VFP3 and VFP5 with Agrobacterium Virulence Protein VirF.en
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2015-11-16
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0142128
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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