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        Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

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        Author
        Goh, Shan
        Ngugi, Daniel
        Lizundia, Regina
        Hostettler, Isabel
        Woods, Kerry
        Ballingall, Keith
        MacHugh, Niall D
        Morrison, W Ivan
        Weir, Willie
        Shiels, Brian
        Werling, Dirk
        Attention
        2299/19784
        Abstract
        As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.
        Publication date
        2016-09-09
        Published in
        PLoS ONE
        Published version
        https://doi.org/10.1371/journal.pone.0162571
        License
        http://creativecommons.org/licenses/by/4.0/
        Other links
        http://hdl.handle.net/2299/19784
        Relations
        School of Life and Medical Sciences
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