Show simple item record

dc.contributor.authorMondhe, Madhav
dc.contributor.authorChessher, Ashley
dc.contributor.authorGoh, Shan
dc.contributor.authorGood, Liam
dc.contributor.authorStach, James E M
dc.date.accessioned2018-02-16T16:18:19Z
dc.date.available2018-02-16T16:18:19Z
dc.date.issued2014-02-18
dc.identifier.citationMondhe , M , Chessher , A , Goh , S , Good , L & Stach , J E M 2014 , ' Species-selective killing of bacteria by antimicrobial peptide-PNAs ' , PLoS ONE , vol. 9 , no. 2 , pp. e89082 . https://doi.org/10.1371/journal.pone.0089082
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 11775430
dc.identifier.otherPURE UUID: 113bc2f1-a475-4ff8-9901-2e1b4d271069
dc.identifier.otherPubMed: 24558473
dc.identifier.otherPubMedCentral: PMC3928365
dc.identifier.otherScopus: 84895868996
dc.identifier.otherORCID: /0000-0002-9028-0303/work/62751718
dc.identifier.urihttp://hdl.handle.net/2299/19788
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License, CC BY 4.0 which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractBroad-spectrum antimicrobials kill indiscriminately, a property that can lead to negative clinical consequences and an increase in the incidence of resistance. Species-specific antimicrobials that could selectively kill pathogenic bacteria without targeting other species in the microbiome could limit these problems. The pathogen genome presents an excellent target for the development of such antimicrobials. In this study we report the design and evaluation of species-selective peptide nucleic acid (PNA) antibacterials. Selective growth inhibition of B. subtilis, E. coli, K. pnuemoniae and S. enterica serovar Typhimurium in axenic or mixed culture could be achieved with PNAs that exploit species differences in the translation initiation region of essential genes. An S. Typhimurium-specific PNA targeting ftsZ resulted in elongated cells that were not observed in E. coli, providing phenotypic evidence of the selectivity of PNA-based antimicrobials. Analysis of the genomes of E. coli and S. Typhimurium gave a conservative estimate of >150 PNA targets that could potentially discriminate between these two closely related species. This work provides a basis for the development of a new class of antimicrobial with a tuneable spectrum of activity.en
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.rightsOpen
dc.subjectAnti-Infective Agents
dc.subjectAntimicrobial Cationic Peptides
dc.subjectBacillus subtilis
dc.subjectBacteria
dc.subjectEscherichia coli
dc.subjectKlebsiella pneumoniae
dc.subjectMicrobial Sensitivity Tests
dc.subjectMicroscopy, Fluorescence
dc.subjectPeptide Nucleic Acids
dc.subjectSalmonella typhimurium
dc.subjectSpecies Specificity
dc.subjectJournal Article
dc.subjectResearch Support, Non-U.S. Gov't
dc.titleSpecies-selective killing of bacteria by antimicrobial peptide-PNAsen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2014-02-18
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0089082
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record