dc.contributor.author | Markostamou, Ioanna | |
dc.contributor.author | Ioannidis, Anestis | |
dc.contributor.author | Dandi, Evgenia | |
dc.contributor.author | Mandyla, Maria Aikaterini | |
dc.contributor.author | Nousiopoulou, Evangelia | |
dc.contributor.author | Simeonidou, Constantina | |
dc.contributor.author | Spandou, Evangelia | |
dc.contributor.author | Tata, Despina A. | |
dc.date.accessioned | 2018-02-28T17:04:29Z | |
dc.date.available | 2018-02-28T17:04:29Z | |
dc.date.issued | 2016-08-01 | |
dc.identifier.citation | Markostamou , I , Ioannidis , A , Dandi , E , Mandyla , M A , Nousiopoulou , E , Simeonidou , C , Spandou , E & Tata , D A 2016 , ' Maternal separation prior to neonatal hypoxia-ischemia : Impact on emotional aspects of behavior and markers of synaptic plasticity in hippocampus ' , International Journal of Developmental Neuroscience , vol. 52 , pp. 1-12 . https://doi.org/10.1016/j.ijdevneu.2016.04.002 | |
dc.identifier.issn | 0736-5748 | |
dc.identifier.other | ORCID: /0000-0001-7343-0122/work/128033146 | |
dc.identifier.uri | http://hdl.handle.net/2299/19840 | |
dc.description | This document is the Accepted Manuscript version, made available under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License CC BY NC-ND 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/ ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. The Version of Record is available online at doi: https://doi.org/10.1016/j.ijdevneu.2016.04.002. | |
dc.description.abstract | Exposure to early-life stress is associated with long-term alterations in brain and behavior, and may aggravate the outcome of neurological insults. This study aimed at investigating the possible interaction between maternal separation, a model of early stress, and subsequent neonatal hypoxia-ischemia on emotional behavior and markers of synaptic plasticity in hippocampus. Therefore, rat pups (N = 60) were maternally separated for a prolonged (MS 180min) or a brief (MS 15min) period during the first six postnatal days, while a control group was left undisturbed. Hypoxia-ischemia was applied to a subgroup of each rearing condition on postnatal day 7. Emotional behavior was examined at three months of age and included assessments of anxiety (elevated plus maze), depression-like behavior (forced swimming) and spontaneous exploration (open field). Synaptic plasticity was evaluated based on BDNF and synaptophysin expression in CA3 and dentate gyrus hippocampal regions. We found that neonatal hypoxia-ischemia caused increased levels of anxiety, depression-like behavior and locomotor activity (ambulation). Higher anxiety levels were also seen in maternally separated rats (MS180min) compared to non-maternally separated rats, but prolonged maternal separation prior to HI did not potentiate the HI-associated effect. No differences among the three rearing conditions were found regarding depression-like behavior or ambulation. Immunohistochemical evaluation of synaptophysin revealed that both prolonged maternal separation (MS180min) and neonatal hypoxia-ischemia significantly reduced its expression in the CA3 and dentate gyrus. Decreases in synaptophysin expression in these areas were not exacerbated in rats that were maternally separated for a prolonged period prior to HI. Regarding BDNF expression, we found a significant decrease in immunoreactivity only in the hypoxic-ischemic rats that were subjected to the prolonged maternal separation paradigm. The above findings suggest that early-life stress prior to neonatal hypoxia-ischemia leads to significant alterations in synaptic plasticity of the dorsal hippocampus during adulthood, but does not exacerbate HI-related changes in emotional behavior. | en |
dc.format.extent | 12 | |
dc.format.extent | 2572558 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Developmental Neuroscience | |
dc.subject | Anxiety | |
dc.subject | BDFN | |
dc.subject | Depression-like behavior | |
dc.subject | Neonatal hypoxia-ischemia | |
dc.subject | Neonatal stress | |
dc.subject | Synaptophysin | |
dc.subject | Developmental Neuroscience | |
dc.subject | Developmental Biology | |
dc.title | Maternal separation prior to neonatal hypoxia-ischemia : Impact on emotional aspects of behavior and markers of synaptic plasticity in hippocampus | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Psychology and Sports Sciences | |
dc.contributor.institution | Centre for Research in Psychology and Sport Sciences | |
dc.description.status | Peer reviewed | |
dc.date.embargoedUntil | 2017-05-07 | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=84973334747&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1016/j.ijdevneu.2016.04.002 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |