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dc.contributor.authorBrennan, Des
dc.contributor.authorCoughlan, Helena
dc.contributor.authorClancy, Eoin
dc.contributor.authorDimov, Nikolay
dc.contributor.authorBarry, Thomas
dc.contributor.authorKinahan, David
dc.contributor.authorDucrée, Jens
dc.contributor.authorSmith, Terry J.
dc.contributor.authorGalvin, Paul
dc.date.accessioned2018-03-12T12:57:03Z
dc.date.available2018-03-12T12:57:03Z
dc.date.issued2017-02-01
dc.identifier.citationBrennan , D , Coughlan , H , Clancy , E , Dimov , N , Barry , T , Kinahan , D , Ducrée , J , Smith , T J & Galvin , P 2017 , ' Development of an on-disc isothermal in vitro amplification and detection of bacterial RNA ' , Sensors and Actuators, B: Chemical , vol. 239 , pp. 235-242 . https://doi.org/10.1016/j.snb.2016.08.018
dc.identifier.issn0925-4005
dc.identifier.otherPURE: 13219697
dc.identifier.otherPURE UUID: e8ed62b3-2288-428e-b979-391b6a1a0873
dc.identifier.otherScopus: 84982854033
dc.identifier.otherORCID: /0000-0002-2873-1505/work/63687432
dc.identifier.urihttp://hdl.handle.net/2299/19880
dc.descriptionThis document is the Accepted Manuscript version, made available under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License CC BY NC-ND 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/). The final, published version is available online at doi: https://doi.org/10.1016/j.snb.2016.08.018. Published by Elsevier B. V.
dc.description.abstractWe present a centrifugal microfluidic “Lab-on-a-Disc” (LoaD) system capable of implementing nucleic acid in vitro amplification using non-contact heating and fluorescence detection. The system functionality is verified by implementing a Nucleic Acid Sequence Based Amplification (NASBA) reaction, targeting the tmRNA transcript of Haemophilus influenzae. The NASBA assay incorporates fluorescent molecular beacon probes reporting target tmRNA amplification for endpoint detection. The system implements non-contact IR heating to heat the NASBA reaction to the required target temperatures during denaturation and amplification steps. The LoaD control system facilitates spin speed and chamber positioning for heating and fluorescence detection. The LoaD alignment system uses magnetic fields to locate and lock the chamber in the required position (heating or detection). The NASBA assay was implemented on the system using Haemophilus influenzae tmRNA over the range 102–104 cell equivalent (CE) units. For comparison, identical qNASBA assays were implemented on a Roche LightCycler 2.0 over this concentration range.en
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.rightsEmbargoed
dc.subjectFluorescence detection
dc.subjectIR heating
dc.subjectIsothermal amplification
dc.subjectLab-on-a-Disc(LoaD)
dc.subjecttmRNA
dc.subjectElectronic, Optical and Magnetic Materials
dc.subjectInstrumentation
dc.subjectCondensed Matter Physics
dc.subjectSurfaces, Coatings and Films
dc.subjectMetals and Alloys
dc.subjectMaterials Chemistry
dc.subjectElectrical and Electronic Engineering
dc.titleDevelopment of an on-disc isothermal in vitro amplification and detection of bacterial RNAen
dc.contributor.institutionSchool of Physics, Engineering & Computer Science
dc.description.statusPeer reviewed
dc.date.embargoedUntil2017-08-03
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84982854033&partnerID=8YFLogxK
dc.relation.schoolSchool of Physics, Engineering & Computer Science
dc.description.versiontypeFinal Accepted Version
dcterms.dateAccepted2017-02-01
rioxxterms.versionAM
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.snb.2016.08.018
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeEmbargoed


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