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dc.contributor.authorFindling, Simone
dc.contributor.authorStotz, Henrik
dc.contributor.authorZoeller, Maria
dc.contributor.authorKrischke, Markus
dc.contributor.authorZander, Mark
dc.contributor.authorGatz, Christiane
dc.contributor.authorBerger, Susanne
dc.contributor.authorMueller, Martin J.
dc.date.accessioned2018-05-25T15:56:31Z
dc.date.available2018-05-25T15:56:31Z
dc.date.issued2018-04-02
dc.identifier.citationFindling , S , Stotz , H , Zoeller , M , Krischke , M , Zander , M , Gatz , C , Berger , S & Mueller , M J 2018 , ' TGA2 signaling in response to reactive electrophile species is not dependent on cysteine modification of TGA2 ' , PLoS ONE , vol. 13 , no. 4 , e0195398 . https://doi.org/10.1371/journal.pone.0195398
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 13657115
dc.identifier.otherPURE UUID: 2afbee11-4081-4d4c-b689-ecde983946b0
dc.identifier.otherScopus: 85044856074
dc.identifier.urihttp://hdl.handle.net/2299/20096
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractReactive electrophile species (RES), including prostaglandins, phytoprostanes and 12-oxo phytodienoic acid (OPDA), activate detoxification responses in plants and animals. However, the pathways leading to the activation of defense reactions related to abiotic or biotic stress as a function of RES formation, accumulation or treatment are poorly understood in plants. Here, the thiol-modification of proteins, including the RES-activated basic region/leucine zipper transcription factor TGA2, was studied. TGA2 contains a single cysteine residue (Cys186) that was covalently modified by reactive cyclopentenones but not required for induction of detoxification genes in response to OPDA or prostaglandin A1. Activation of the glutathione-S-transferase 6 (GST6) promoter was responsive to cyclopentenones but not to unreactive cyclopentanones, including jasmonic acid suggesting that thiol reactivity of RES is important to activate the TGA2-dependent signaling pathway resulting in GST6 activation We show that RES modify thiols in numerous proteins in vivo, however, thiol reactivity alone appears not to be sufficient for biological activity as demonstrated by the failure of several membrane permeable thiol reactive reagents to activate the GST6 promoter.en
dc.format.extent21
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.rightsOpen
dc.subjectAmino Acids/pharmacology
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins/chemistry
dc.subjectBasic-Leucine Zipper Transcription Factors/chemistry
dc.subjectCyclopentanes/pharmacology
dc.subjectCysteine/metabolism
dc.subjectEscherichia coli
dc.subjectFatty Acids, Unsaturated/pharmacology
dc.subjectGlutathione Transferase/genetics
dc.subjectNuclear Proteins/chemistry
dc.subjectOxylipins/pharmacology
dc.subjectPipecolic Acids/pharmacology
dc.subjectPlant Leaves/drug effects
dc.subjectPlants, Genetically Modified
dc.subjectPromoter Regions, Genetic
dc.subjectProstaglandins A/pharmacology
dc.subjectRecombinant Proteins/metabolism
dc.subjectSeedlings/drug effects
dc.subjectSignal Transduction/drug effects
dc.subjectAgricultural and Biological Sciences(all)
dc.subjectBiochemistry, Genetics and Molecular Biology(all)
dc.titleTGA2 signaling in response to reactive electrophile species is not dependent on cysteine modification of TGA2en
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionExtracellular Vesicle Research Unit
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85044856074&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2018-04-02
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0195398
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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