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dc.contributor.authorStotz, Henrik
dc.contributor.authorPascoe, Harvey
dc.contributor.authorParham, Haddadi
dc.contributor.authorFitt, Bruce
dc.contributor.authorMashanova, Alla
dc.contributor.authorKukol, Andreas
dc.contributor.authorLarkan, Nicholas
dc.contributor.authorHossein, Borhan
dc.date.accessioned2018-07-03T16:24:13Z
dc.date.available2018-07-03T16:24:13Z
dc.date.issued2018-06-01
dc.identifier.citationStotz , H , Pascoe , H , Parham , H , Fitt , B , Mashanova , A , Kukol , A , Larkan , N & Hossein , B 2018 , ' Genomic evidence for genes encoding leucine-rich repeat receptors linked to resistance against the eukaryotic extra- and intracellular Brassica napus pathogens Leptosphaeria maculans and Plasmodiophora brassicae ' , PLoS ONE , vol. 13 , no. 6 , e0198201 , pp. 1-17 . https://doi.org/10.1371/journal.pone.0198201
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 13867171
dc.identifier.otherPURE UUID: 383d2e29-753d-4f45-a0a2-14dc7654b319
dc.identifier.otherScopus: 85048053621
dc.identifier.otherORCID: /0000-0003-3273-8184/work/74071752
dc.identifier.urihttp://hdl.handle.net/2299/20248
dc.description© 2018 Stotz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractGenes coding for nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) control resistance against intracellular (cell-penetrating) pathogens. However, evidence for a role of genes coding for proteins with LRR domains in resistance against extracellular (apoplastic) fungal pathogens is limited. Here, the distribution of genes coding for proteins with eLRR domains but lacking kinase domains was determined for the Brassica napus genome. Predictions of signal peptide and transmembrane regions divided these genes into 184 coding for receptor-like proteins (RLPs) and 121 coding for secreted proteins (SPs). Together with previously annotated NLRs, a total of 720 LRR genes were found. Leptosphaeria maculans-induced expression during a compatible interaction with cultivar Topas differed between RLP, SP and NLR gene families; NLR genes were induced relatively late, during the necrotrophic phase of pathogen colonization. Seven RLP, one SP and two NLR genes were found in Rlm1 and Rlm3/Rlm4/Rlm7/Rlm9 loci for resistance against L. maculans on chromosome A07 of B. napus. One NLR gene at the Rlm9 locus was positively selected, as was the RLP gene on chromosome A10 with LepR3 and Rlm2 alleles conferring resistance against L. maculans races with corresponding effectors AvrLm1 and AvrLm2, respectively. Known loci for resistance against L. maculans (extracellular hemi-biotrophic fungus), Sclerotinia sclerotiorum (necrotrophic fungus) and Plasmodiophora brassicae (intracellular, obligate biotrophic protist) were examined for presence of RLPs, SPs and NLRs in these regions. Whereas loci for resistance against P. brassicae were enriched for NLRs, no such signature was observed for the other pathogens. These findings demonstrate involvement of (i) NLR genes in resistance against the intracellular pathogen P. brassicae and a putative NLR gene in Rlm9-mediated resistance against the extracellular pathogen L. maculans.en
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.subjectAscomycota/physiology
dc.subjectBrassica napus/genetics
dc.subjectDisease Resistance/genetics
dc.subjectGenes, Plant
dc.subjectGenome, Plant
dc.subjectGenome-Wide Association Study
dc.subjectHost-Parasite Interactions/genetics
dc.subjectModels, Molecular
dc.subjectMultigene Family
dc.subjectPhylogeny
dc.subjectPlant Cells/microbiology
dc.subjectPlant Diseases/genetics
dc.subjectPlant Proteins/chemistry
dc.subjectPlasmodiophorida/physiology
dc.subjectProtein Conformation
dc.subjectProteins/chemistry
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.titleGenomic evidence for genes encoding leucine-rich repeat receptors linked to resistance against the eukaryotic extra- and intracellular Brassica napus pathogens Leptosphaeria maculans and Plasmodiophora brassicaeen
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionEcology
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionWeight and Obesity Research Group
dc.contributor.institutionExtracellular Vesicle Research Unit
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85048053621&partnerID=8YFLogxK
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0198201
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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