dc.contributor.author | Stratton, Dan | |
dc.contributor.author | Inal, Jameel | |
dc.date.accessioned | 2018-07-31T12:50:47Z | |
dc.date.available | 2018-07-31T12:50:47Z | |
dc.date.issued | 2012-09-13 | |
dc.identifier.citation | Stratton , D & Inal , J 2012 , ' Characterisation of microvesicles released from cells constitutively and upon stimulation ' , Microvesiculation and Disease, a Biochemical Society Focused Meeting , London , United Kingdom , 13/09/12 - 14/09/12 . | |
dc.identifier.citation | conference | |
dc.identifier.uri | http://hdl.handle.net/2299/20308 | |
dc.description.abstract | Constitutively released microvesicles (cMVs) are released as a part of normal cell physiology and this is summarised in Fig. 1 (1). However stimulated microvesicles (sMVs) are released as a result of a number of possible stress factors (2, 3). We found sMVs to be released in higher numbers than cMVs, typically ten-fold higher numbers, in the same time frame, and where the stress factor was a pharmacological agent, the microvesiculation was an attempt to release this chemical stress factor. Using a mass sensing technique, the sMVs were released over a 15 min period after stimulation. Using sizing beads on a flow cytometer and by transmission electron microscopy the cMVs were typically smaller (less than 300 nm in diameter) than sMVs (300-500 nm in diameter). However cMVs were found to carry more protein. By contrast, phosphatidylserine expression was greater on the larger sMVs, which also more effectively inhibited complement-mediated lysis. | en |
dc.format.extent | 104776 | |
dc.language.iso | eng | |
dc.title | Characterisation of microvesicles released from cells constitutively and upon stimulation | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Biological and Environmental Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.description.status | Peer reviewed | |
rioxxterms.type | Other | |
herts.preservation.rarelyaccessed | true | |