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dc.contributor.authorBraoudaki, Maria
dc.contributor.authorLambrou
dc.contributor.authorPapadodima
dc.contributor.authorStefanaki
dc.contributor.authorProdromou
dc.contributor.authorKanavakis
dc.date.accessioned2018-11-17T02:14:58Z
dc.date.available2018-11-17T02:14:58Z
dc.date.issued2016-01-30
dc.identifier.citationBraoudaki , M , Lambrou , Papadodima , Stefanaki , Prodromou & Kanavakis 2016 , ' MicroRNA expression profiles in pediatric dysembryoplastic neuroepithelial tumors. ' , Medical Oncology , vol. 33 , no. 5 , pp. 1-7 . https://doi.org/10.1007/s12032-015-0719-3
dc.identifier.issn1559-131X
dc.identifier.urihttp://hdl.handle.net/2299/20801
dc.description© Springer Science+Business Media New York 2015
dc.description.abstractAmong noncoding RNAs, microRNAs (miRNAs) have been most extensively studied, and their biology has repeatedly been proven critical for central nervous system pathological conditions. The diagnostic value of several miRNAs was appraised in pediatric dysembryoplastic neuroepithelial tumors (DNETs) using miRNA microarrays and receiving operating characteristic curves analyses. Overall, five pediatric DNETs were studied. As controls, 17 samples were used: the FirstChoice Human Brain Reference RNA and 16 samples from deceased children who underwent autopsy and were not present with any brain malignancy. The miRNA extraction was carried out using the mirVANA miRNA Isolation Kit, while the experimental approach included miRNA microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction was performed to validate the expression profiles of miR-1909* and miR-3138 in all samples initially screened with miRNA microarrays. Our findings indicated that miR-3138 might act as a tumor suppressor gene when down-regulated and miR-1909* as a putative oncogenic molecule when up-regulated in pediatric DNETs compared to the control cohort. Subsequently, both miRNA signatures might serve as putative diagnostic biomarkers for pediatric DNETs.en
dc.format.extent7
dc.format.extent847892
dc.language.isoeng
dc.relation.ispartofMedical Oncology
dc.subjectpediatric
dc.subjectbrain tumours
dc.subjectmicroRNA
dc.subjectmicroarrays
dc.subjectqRT-PCR
dc.subjectBiomarkers
dc.subjectROC curves
dc.titleMicroRNA expression profiles in pediatric dysembryoplastic neuroepithelial tumors.en
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionCentre for Research in Mechanisms of Disease and Drug Discovery
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Future Societies Research
dc.description.statusPeer reviewed
dc.date.embargoedUntil2016-12-23
rioxxterms.versionofrecord10.1007/s12032-015-0719-3
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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