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dc.contributor.authorPhani, Victor
dc.contributor.authorSomvanshi, Vishal
dc.contributor.authorShukla, Rohit
dc.contributor.authorDavies, Keith
dc.contributor.authorRao, Uma
dc.date.accessioned2018-12-20T10:12:28Z
dc.date.available2018-12-20T10:12:28Z
dc.date.issued2018-11-29
dc.identifier.citationPhani , V , Somvanshi , V , Shukla , R , Davies , K & Rao , U 2018 , ' A transcriptomic snapshot of early molecular communication between Pasteuria penetrans and Meloidogyne incognita ' , BMC Genomics , vol. 19 , no. 1 , 850 , pp. 1-15 . https://doi.org/10.1186/s12864-018-5230-8
dc.identifier.issn1471-2164
dc.identifier.otherPURE: 15692914
dc.identifier.otherPURE UUID: cbd37d1b-ca86-49b3-82a2-1139fe2fca48
dc.identifier.otherScopus: 85057556346
dc.identifier.urihttp://hdl.handle.net/2299/20880
dc.description© The Author(s). 2018
dc.description.abstractBackground: Southern root-knot nematode Meloidogyne incognita (Kofoid and White, 1919), Chitwood, 1949 is a key pest of agricultural crops. Pasteuria penetrans is a hyperparasitic bacterium capable of suppressing the nematode reproduction, and represents a typical coevolved pathogen-hyperparasite system. Attachment of Pasteuria endospores to the cuticle of second-stage nematode juveniles is the first and pivotal step in the bacterial infection. RNA-Seq was used to understand the early transcriptional response of the root-knot nematode at 8 h post Pasteuria endospore attachment. Results: A total of 52,485 transcripts were assembled from the high quality (HQ) reads, out of which 582 transcripts were found differentially expressed in the Pasteuria endospore encumbered J2 s, of which 229 were up-regulated and 353 were down-regulated. Pasteuria infection caused a suppression of the protein synthesis machinery of the nematode. Several of the differentially expressed transcripts were putatively involved in nematode innate immunity, signaling, stress responses, endospore attachment process and post-attachment behavioral modification of the juveniles. The expression profiles of fifteen selected transcripts were validated to be true by the qRT PCR. RNAi based silencing of transcripts coding for fructose bisphosphate aldolase and glucosyl transferase caused a reduction in endospore attachment as compared to the controls, whereas, silencing of aspartic protease and ubiquitin coding transcripts resulted in higher incidence of endospore attachment on the nematode cuticle. Conclusions: Here we provide evidence of an early transcriptional response by the nematode upon infection by Pasteuria prior to root invasion. We found that adhesion of Pasteuria endospores to the cuticle induced a down-regulated protein response in the nematode. In addition, we show that fructose bisphosphate aldolase, glucosyl transferase, aspartic protease and ubiquitin coding transcripts are involved in modulating the endospore attachment on the nematode cuticle. Our results add new and significant information to the existing knowledge on early molecular interaction between M. incognita and P. penetrans.en
dc.format.extent15
dc.language.isoeng
dc.relation.ispartofBMC Genomics
dc.rights/dk/atira/pure/core/openaccesspermission/open
dc.subjectEndospores
dc.subjectMeloidogyne incognita
dc.subjectPasteuria penetrans
dc.subjectRoot-knot nematode
dc.subjectTranscriptome
dc.subjectBiotechnology
dc.subjectGenetics
dc.titleA transcriptomic snapshot of early molecular communication between Pasteuria penetrans and Meloidogyne incognitaen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85057556346&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2018-11-07
rioxxterms.versionP
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1186/s12864-018-5230-8
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeopenAccess


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