dc.contributor.author | Phani, Victor | |
dc.contributor.author | Shivakumra, Tagginahalli | |
dc.contributor.author | Davies, Keith | |
dc.contributor.author | Rao, Uma | |
dc.date.accessioned | 2018-12-20T11:00:35Z | |
dc.date.available | 2018-12-20T11:00:35Z | |
dc.date.issued | 2018-11-01 | |
dc.identifier.citation | Phani , V , Shivakumra , T , Davies , K & Rao , U 2018 , ' Knockdown of a mucin-like gene in Meloidogyne incognita (Nematoda) decreases attachment of endospores of Pasteuria penetrans to the infective juveniles and reduces nematode fecundity : Mucin-like gene from M. incognita ' , Molecular Plant Pathology , vol. 19 , no. 11 , pp. 2370-2383 . https://doi.org/10.1111/mpp.12704 | |
dc.identifier.issn | 1464-6722 | |
dc.identifier.other | ORCID: /0000-0001-6060-2394/work/62750575 | |
dc.identifier.uri | http://hdl.handle.net/2299/20884 | |
dc.description.abstract | Mucins are highly glycosylated polypeptides involved in many host–parasite interactions, but their function in plant-parasitic nematodes is still unknown. In this study, a mucin-like gene was cloned from Meloidogyne incognita (Mi-muc-1, 1125 bp) and characterized. The protein was found to be rich in serine and threonine with numerous O-glycosylation sites in the sequence. Quantitative real-time polymerase chain reaction (qRT-PCR) showed the highest expression in the adult female and in situ hybridization revealed the localization of Mi-muc-1 mRNA expression in the tail area in the region of the phasmid. Knockdown of Mi-muc-1 revealed a dual role: (1) immunologically, there was a significant decrease in attachment of Pasteuria penetrans endospores and a reduction in binding assays with human red blood cells (RBCs), suggesting that Mi-MUC-1 is a glycoprotein present on the surface coat of infective second-stage juveniles (J2s) and is involved in cellular adhesion to the cuticle of infective J2s; pretreatment of J2s with different carbohydrates indicated that the RBCs bind to J2 cuticle receptors different from those involved in the interaction of Pasteuria endospores with Mi-MUC-1; (2) the long-term effect of RNA interference (RNAi)-mediated knockdown of Mi-muc-1 led to a significant reduction in nematode fecundity, suggesting a possible function for this mucin as a mediator in the interaction between the nematode and the host plant. | en |
dc.format.extent | 14 | |
dc.format.extent | 690144 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular Plant Pathology | |
dc.subject | attachment | |
dc.subject | fecundity | |
dc.subject | glycocalyx | |
dc.subject | immunity | |
dc.subject | M. incognita | |
dc.subject | mucin | |
dc.subject | P. penetrans | |
dc.subject | Molecular Biology | |
dc.subject | Agronomy and Crop Science | |
dc.subject | Soil Science | |
dc.subject | Plant Science | |
dc.title | Knockdown of a mucin-like gene in Meloidogyne incognita (Nematoda) decreases attachment of endospores of Pasteuria penetrans to the infective juveniles and reduces nematode fecundity : Mucin-like gene from M. incognita | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Biological and Environmental Sciences | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Geography, Environment and Agriculture | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85051660616&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1111/mpp.12704 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |