Show simple item record

dc.contributor.authorShah, Unnati
dc.contributor.authorFitt, Bruce
dc.contributor.authorKotta-Loizou, Ioly
dc.contributor.authorCoutts, Robert
dc.date.accessioned2019-01-16T13:00:12Z
dc.date.available2019-01-16T13:00:12Z
dc.date.issued2018-12-25
dc.identifier.citationShah , U , Fitt , B , Kotta-Loizou , I & Coutts , R 2018 , ' Identification, Molecular Characterization, and Biology of a Novel Quadrivirus Infecting the Phytopathogenic Fungus Leptosphaeria biglobosa. ' , Viruses , vol. 11 , no. 1 , 9 . https://doi.org/10.3390/v11010009
dc.identifier.issn1999-4915
dc.identifier.otherPURE: 16097499
dc.identifier.otherPURE UUID: 13c2cd47-f2fd-4c0a-b8cc-cb405ee89a23
dc.identifier.otherScopus: 85059119568
dc.identifier.urihttp://hdl.handle.net/2299/20969
dc.description.abstractHere we report the molecular characterisation of a novel dsRNA virus isolated from the filamentous, plant pathogenic fungus Leptosphaeria biglobosa and known to cause significant alterations to fungal pigmentation and growth and to result in hypervirulence, as illustrated by comparisons between virus-infected and-cured isogenic fungal strains. The virus forms isometric particles approximately 40–45 nm in diameter and has a quadripartite dsRNA genome structure with size ranges of 4.9 to 4 kbp, each possessing a single ORF. Sequence analysis of the putative proteins encoded by dsRNAs 1–4, termed P1–P4, respectively, revealed modest similarities to the amino acid sequences of equivalent proteins predicted from the nucleotide sequences of known and suspected members of the family Quadriviridae and for that reason the virus was nominated Leptosphaeria biglobosa quadrivirus-1 (LbQV-1). Sequence and phylogenetic analysis using the P3 sequence, which encodes an RdRP, revealed that LbQV-1 was most closely related to known and suspected quadriviruses and monopartite totiviruses rather than other quadripartite mycoviruses including chrysoviruses and alternaviruses. Of the remaining encoded proteins, LbQV-1 P2 and P4 are structural proteins but the function of P1 is unknown. We propose that LbQV-1 is a novel member of the family Quadriviridae.en
dc.language.isoeng
dc.relation.ispartofViruses
dc.subjectDsRNA mycoviruses
dc.subjectFungal viruses
dc.subjectHypervirulence
dc.subjectLeptosphaeria biglobosa quadrivirus
dc.subjectAgricultural and Biological Sciences (miscellaneous)
dc.subjectAgronomy and Crop Science
dc.subjectPlant Science
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectMolecular Biology
dc.subjectVirology
dc.subjectInfectious Diseases
dc.titleIdentification, Molecular Characterization, and Biology of a Novel Quadrivirus Infecting the Phytopathogenic Fungus Leptosphaeria biglobosa.en
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionWeight and Obesity Research Group
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85059119568&partnerID=8YFLogxK
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.3390/v11010009
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record