dc.contributor.author | Hastoy, Benoît | |
dc.contributor.author | Godazgar, Mahdieh | |
dc.contributor.author | Clark, Anne | |
dc.contributor.author | Nylander, Vibe | |
dc.contributor.author | Spiliotis, Ioannis | |
dc.contributor.author | van de Bunt, Martijn | |
dc.contributor.author | Chibalina, Margarita V | |
dc.contributor.author | Barrett, Amy | |
dc.contributor.author | Burrows, Carla | |
dc.contributor.author | Tarasov, Andrei I | |
dc.contributor.author | Scharfmann, Raphael | |
dc.contributor.author | Gloyn, Anna L | |
dc.contributor.author | Rorsman, Patrik | |
dc.date.accessioned | 2019-03-05T11:18:14Z | |
dc.date.available | 2019-03-05T11:18:14Z | |
dc.date.issued | 2018-11-19 | |
dc.identifier.citation | Hastoy , B , Godazgar , M , Clark , A , Nylander , V , Spiliotis , I , van de Bunt , M , Chibalina , M V , Barrett , A , Burrows , C , Tarasov , A I , Scharfmann , R , Gloyn , A L & Rorsman , P 2018 , ' Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells ' , Scientific Reports , vol. 8 , no. 1 , 16994 , pp. 1-16 . https://doi.org/10.1038/s41598-018-34743-7 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | ORCID: /0000-0002-8883-176X/work/159376003 | |
dc.identifier.uri | http://hdl.handle.net/2299/21165 | |
dc.description | © The Author(s) 2018 | |
dc.description.abstract | Limited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-βH1 and EndoC-βH2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters that constitute the glucose-triggering pathway of insulin release. Both cell lines respond to glucose (6 and 20 mM) with 2- to 3-fold stimulation of insulin secretion which correlated with an elevation of [Ca2+]i, membrane depolarisation and increased action potential firing. Similar to human primary beta cells, KATP channel activity is low at 1 mM glucose and is further reduced upon increasing glucose concentration; an effect that was mimicked by the KATP channel blocker tolbutamide. The upstroke of the action potentials reflects the activation of Ca2+ channels with some small contribution of TTX-sensitive Na+ channels. The repolarisation involves activation of voltage-gated Kv2.2 channels and large-conductance Ca2+-activated K+ channels. Exocytosis presented a similar kinetics to human primary beta cells. The ultrastructure of these cells shows insulin vesicles composed of an electron-dense core surrounded by a thin clear halo. We conclude that the EndoC-βH1 and -βH2 cells share many features of primary human β-cells and thus represent a useful experimental model. | en |
dc.format.extent | 4554030 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | |
dc.subject | General | |
dc.title | Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells | en |
dc.contributor.institution | Department of Biological and Environmental Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85056745036&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1038/s41598-018-34743-7 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |