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dc.contributor.authorHastoy, Benoît
dc.contributor.authorGodazgar, Mahdieh
dc.contributor.authorClark, Anne
dc.contributor.authorNylander, Vibe
dc.contributor.authorSpiliotis, Ioannis
dc.contributor.authorvan de Bunt, Martijn
dc.contributor.authorChibalina, Margarita V
dc.contributor.authorBarrett, Amy
dc.contributor.authorBurrows, Carla
dc.contributor.authorTarasov, Andrei I
dc.contributor.authorScharfmann, Raphael
dc.contributor.authorGloyn, Anna L
dc.contributor.authorRorsman, Patrik
dc.date.accessioned2019-03-05T11:18:14Z
dc.date.available2019-03-05T11:18:14Z
dc.date.issued2018-11-19
dc.identifier.citationHastoy , B , Godazgar , M , Clark , A , Nylander , V , Spiliotis , I , van de Bunt , M , Chibalina , M V , Barrett , A , Burrows , C , Tarasov , A I , Scharfmann , R , Gloyn , A L & Rorsman , P 2018 , ' Electrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cells ' , Scientific Reports , vol. 8 , no. 1 , 16994 , pp. 1-16 . https://doi.org/10.1038/s41598-018-34743-7
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 15699077
dc.identifier.otherPURE UUID: ed4bd133-4720-4f01-8494-bc6887c668d2
dc.identifier.otherPubMed: 30451893
dc.identifier.otherScopus: 85056745036
dc.identifier.urihttp://hdl.handle.net/2299/21165
dc.description© The Author(s) 2018
dc.description.abstractLimited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-βH1 and EndoC-βH2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters that constitute the glucose-triggering pathway of insulin release. Both cell lines respond to glucose (6 and 20 mM) with 2- to 3-fold stimulation of insulin secretion which correlated with an elevation of [Ca2+]i, membrane depolarisation and increased action potential firing. Similar to human primary beta cells, KATP channel activity is low at 1 mM glucose and is further reduced upon increasing glucose concentration; an effect that was mimicked by the KATP channel blocker tolbutamide. The upstroke of the action potentials reflects the activation of Ca2+ channels with some small contribution of TTX-sensitive Na+ channels. The repolarisation involves activation of voltage-gated Kv2.2 channels and large-conductance Ca2+-activated K+ channels. Exocytosis presented a similar kinetics to human primary beta cells. The ultrastructure of these cells shows insulin vesicles composed of an electron-dense core surrounded by a thin clear halo. We conclude that the EndoC-βH1 and -βH2 cells share many features of primary human β-cells and thus represent a useful experimental model.en
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.rightsOpen
dc.subjectGeneral
dc.titleElectrophysiological properties of human beta-cell lines EndoC-βH1 and -βH2 conform with human beta-cellsen
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85056745036&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2018-11-19
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1038/s41598-018-34743-7
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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