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dc.contributor.authorKosgodage, Uchini S
dc.contributor.authorMatewele, Paul
dc.contributor.authorMastroianni, Giulia
dc.contributor.authorLange, Sigrun
dc.contributor.authorKraev, Igor
dc.contributor.authorBrotherton, Dominik
dc.contributor.authorAwamaria, Brigitte
dc.contributor.authorNicholas, Anthony P.
dc.contributor.authorInal, Jameel
dc.date.accessioned2019-08-14T09:15:27Z
dc.date.available2019-08-14T09:15:27Z
dc.date.issued2019-06-27
dc.identifier.citationKosgodage , U S , Matewele , P , Mastroianni , G , Lange , S , Kraev , I , Brotherton , D , Awamaria , B , Nicholas , A P & Inal , J 2019 , ' Peptidylarginine deiminase inhibitors reduce bacterial membrane vesicle release and sensitize bacteria to antibiotic treatment ' , Frontiers Cellular and Infection Microbiology , vol. 9 , 227 . https://doi.org/10.3389/fcimb.2019.00227
dc.identifier.issn2235-2988
dc.identifier.otherPURE: 16946714
dc.identifier.otherPURE UUID: 41fd3111-969c-40d0-89c7-d61fcfdc3f76
dc.identifier.otherScopus: 85068897821
dc.identifier.urihttp://hdl.handle.net/2299/21593
dc.description.abstractOuter membrane and membrane vesicles (OMV/MV) are released from bacteria and participate in cell communication, biofilm formation and host-pathogen interactions. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that catalyze post-translational deimination/citrullination of proteins, causing structural and functional changes in target proteins. PADs also play major roles in the regulation of eukaryotic extracellular vesicle release. Here we show phylogenetically conserved pathways of PAD-mediated OMV/MV release in bacteria and describe deiminated/citrullinated proteins in E. coli and their derived OMV/MVs. Furthermore, we show that PAD inhibitors can be used to effectively reduce OMV/MV release, both in Gram-negative and Gram-positive bacteria. Importantly, this resulted in enhanced antibiotic sensitivity of both E. coli and S. aureus to a range of antibiotics tested. Our findings reveal novel strategies for applying pharmacological OMV/MV-inhibition to reduce antibiotic resistance.en
dc.language.isoeng
dc.relation.ispartofFrontiers Cellular and Infection Microbiology
dc.rightsOpen
dc.subjectAntibiotic sensitivity
dc.subjectDeimination/citrullination
dc.subjectE. coli VCS257
dc.subjectOuter-membrane vesicles (OMVs)
dc.subjectPeptidylarginine deiminase (PAD)
dc.subjectS. aureus subsp. aureus Rosenbach
dc.subjectMicrobiology
dc.subjectImmunology
dc.subjectMicrobiology (medical)
dc.subjectInfectious Diseases
dc.titlePeptidylarginine deiminase inhibitors reduce bacterial membrane vesicle release and sensitize bacteria to antibiotic treatmenten
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionExtracellular Vesicle Research Unit
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85068897821&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2019-06-27
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.3389/fcimb.2019.00227
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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