dc.contributor.author | Lione, Lisa | |
dc.contributor.author | Benham, Christopher | |
dc.contributor.author | Shamsaldeen, Yousif | |
dc.date.accessioned | 2019-09-05T10:25:06Z | |
dc.date.available | 2019-09-05T10:25:06Z | |
dc.date.issued | 2019-08-13 | |
dc.identifier.citation | Lione , L , Benham , C & Shamsaldeen , Y 2019 , Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin-1 and endothelial dysfunction in streptozotocin treated rats : Supplement to Diabetes Metab Res Rev. 34(5):e2995. . Biomedical Advances . < http://biomedical-advances.org/diabetes-201905/ > | |
dc.identifier.uri | http://hdl.handle.net/2299/21658 | |
dc.description.abstract | Background: Diabetes is a complex progressive disease characterised by chronic hyperglycaemia and dyslipidaemia associated with endothelial dysfunction. Oxidised LDL (Ox-LDL) is elevated in diabetes and may contribute to endothelial dysfunction. The aim of this study was to relate the serum levels of Ox-LDL with endothelial dysfunction in streptozotocin (STZ)-diabetic rats and to further explore the changes in endothelial nitric oxide synthase (eNOS) and caveolin-1 (CAV-1) expression in primary aortic endothelial cells (ECs).Methods: Diabetes was induced with a single intraperitoneal injection of STZ in male Wistar rats. During the hyperglycaemic diabetes state serum lipid markers, aortic relaxation and aortic ECs eNOS and CAV-1 protein expression was measured.Results: Elevated serum Ox-LDL (STZ 1486 ± 78.1 pg/ml vs control 732.6 ± 160.6pg/ml, p<0.05) was associated with hyperglycaemia (STZ 29 ± 0.9 mmol/L vs control: 7.2 ± 0.2 mmol/L, p<0.001) and hypertriglyceridemia (STZ 9.0 ± 1.5 mmol/L vs control: 3.0 ± 0.3 mmol/L, p<0.01) in diabetic rats. A significant reduction was observed in STZ-diabetic aortic endothelial cell eNOS and CAV-1 of 40% and 30% respectively, accompanied by a compromised STZ-diabetic carbachol-induced vasodilation (STZ 29.6 ± 9.3% vs control 77.2 ± 2.5%, p<0.001).Conclusions: The elevated serum Ox-LDL in hyperglycaemic STZ-diabetic rats may contribute to diabetic endothelial dysfunction, possibly through downregulation of endothelial CAV-1 and eNOS. | en |
dc.format.extent | 554822 | |
dc.language.iso | eng | |
dc.publisher | Biomedical Advances | |
dc.title | Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin-1 and endothelial dysfunction in streptozotocin treated rats : Supplement to Diabetes Metab Res Rev. 34(5):e2995. | en |
dc.contributor.institution | Centre for Health Services and Clinical Research | |
dc.contributor.institution | Basic and Clinical Science Unit | |
dc.contributor.institution | TRP Ion channels | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.identifier.url | http://biomedical-advances.org/diabetes-201905/ | |
rioxxterms.type | Conference Paper/Proceeding/Abstract | |
herts.preservation.rarelyaccessed | true | |