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dc.contributor.authorWilkes, Thomas
dc.contributor.authorWarner, Douglas
dc.contributor.authorEdmonds-Brown, Veronica
dc.contributor.authorDavies, Keith
dc.contributor.authorDenholm, Ian
dc.date.accessioned2019-11-27T01:11:27Z
dc.date.available2019-11-27T01:11:27Z
dc.date.issued2019-11-21
dc.identifier.citationWilkes , T , Warner , D , Edmonds-Brown , V , Davies , K & Denholm , I 2019 , ' A comparison of methodologies for the staining and quantification of intracellular components of Arbuscular Mychorrizal (AM) fungi in the root cortex of two varieties of winter wheat ' , Access Microbiology , pp. 1-6 . https://doi.org/10.1099/acmi.0.000083
dc.identifier.issn2516-8290
dc.identifier.otherPURE: 17669174
dc.identifier.otherPURE UUID: af2a72ad-e201-4862-b029-8d8d7e00f69f
dc.identifier.otherORCID: /0000-0002-9136-9713/work/65312202
dc.identifier.otherORCID: /0000-0001-6060-2394/work/65312399
dc.identifier.otherORCID: /0000-0001-9797-874X/work/65312404
dc.identifier.urihttp://hdl.handle.net/2299/21938
dc.description© 2019 The Authors. The definitive peer reviewed, edited version of this article is published in Access Microbiology, https://doi.org/10.1099/acmi.0.000083. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
dc.description.abstractArbuscular Mychorrizal (AM) fungi are one of the most common fungal organisms to exist in symbiosis with terrestrial plants facilitating the growth and maintenance of arable crops. Wheat has been studied extensively for AM fungal symbiosis using the carcinogen trypan blue as the identifying stain for fungal components, namely arbuscles, vesicles and hyphal structures. The present study uses Sheaffer® blue ink with a lower risk as an alternative to this carcinogenic stain. Justification for this is determined by stained wheat root sections (n = 120), with statistically significant increases in the observed abundance of intracellular root cortical fungal structures stained with Sheaffer® blue ink compared to trypan blue for both Zulu (P = 0.003) and Siskin (P = 0.0003) varieties of winter wheat. This new alternative combines an improved quantification of intracellular fungal components with a lower hazard risk at a lower cost.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofAccess Microbiology
dc.subjectAM fungi
dc.subjectTrypan blue
dc.subjectSheaffer blue
dc.subjectWheat
dc.subjectStaining
dc.titleA comparison of methodologies for the staining and quantification of intracellular components of Arbuscular Mychorrizal (AM) fungi in the root cortex of two varieties of winter wheaten
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionAgriculture and Environment Research Unit
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionWater and Environment
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionEcology
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionAgriculture and Environmental Management Research
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1099/acmi.0.000083
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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