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dc.contributor.authorAntwi-Baffour, Samuel
dc.contributor.authorMalibha-Pinchbeck, Memory
dc.contributor.authorStratton, Dan
dc.contributor.authorJorfi, Samireh
dc.contributor.authorLange, Sigrun
dc.contributor.authorInal, Jameel
dc.date.accessioned2019-12-24T01:05:27Z
dc.date.available2019-12-24T01:05:27Z
dc.date.issued2019-12-18
dc.identifier.citationAntwi-Baffour , S , Malibha-Pinchbeck , M , Stratton , D , Jorfi , S , Lange , S & Inal , J 2019 , ' Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets ' , Journal of Extracellular Vesicles , vol. 9 , no. 1 , 1697124 , pp. 1-13 . https://doi.org/10.1080/20013078.2019.1697124
dc.identifier.issn2001-3078
dc.identifier.otherPURE: 18343229
dc.identifier.otherPURE UUID: 99ecfd09-d663-4060-8943-4946b6202b93
dc.identifier.otherScopus: 85076903602
dc.identifier.urihttp://hdl.handle.net/2299/22031
dc.description© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.description.abstractThis study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, (ii) period from onset of malaria symptoms until seeking medical care (patient delay, PD), (iii) age and (iv) gender. In this cross-sectional study, n = 434 patients were analysed and Nanosight Tracking Analysis (NTA) used to quantify mEVs (vesicles of 150–500 nm diameter, isolated at 15,000 × g, β-tubulin-positive and staining for annexin V, but weak or negative for CD81). Overall plasma mEV levels (1.69 × 10 10 mEVs mL −1) were 2.3-fold higher than for uninfected controls (0.51 × 10 10 mEVs mL −1). Divided into four age groups, we found a bimodal distribution with 2.5- and 2.1-fold higher mEVs in infected children (<11 years old [yo]) (median:2.11 × 10 10 mEVs mL −1) and the elderly (>45 yo) (median:1.92 × 10 10 mEVs mL −1), respectively, compared to uninfected controls; parasite density varied similarly with age groups. There was a positive association between mEVs and parasite density (r = 0.587, p < 0.0001) and mEVs were strongly associated with PD (r = 0.919, p < 0.0001), but gender had no effect on plasma mEV levels (p = 0.667). Parasite density was also exponentially related to patient delay. Gender (p = 0.667) had no effect on plasma mEV levels. During periods of low parasitaemia (PD = 72h), mEVs were 0.93-fold greater than in uninfected controls. As 75% (49/65) of patients had low parasitaemia levels (20–500 parasites µL −1), close to the detection limits of microscopy of Giemsa-stained thick blood films (5–150 parasites µL −1), mEV quantification by NTA could potentially have early diagnostic value, and raises the potential of Pf markers in mEVs as early diagnostic targets.en
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofJournal of Extracellular Vesicles
dc.subjectMalaria
dc.subjectextracellular vesicles
dc.subjectparasitaemia
dc.subjectHistology
dc.subjectCell Biology
dc.titlePlasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targetsen
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionExtracellular Vesicle Research Unit
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85076903602&partnerID=8YFLogxK
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1080/20013078.2019.1697124
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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