dc.contributor.author | Dimov, Nikolay | |
dc.contributor.author | McDonnell, M.B. | |
dc.contributor.author | Munro, Ian | |
dc.contributor.author | McCluskey, Daniel | |
dc.contributor.author | Johnston, Ian | |
dc.contributor.author | Tan, Christabel | |
dc.contributor.author | Coudron, Loic | |
dc.date.accessioned | 2020-02-28T01:07:03Z | |
dc.date.available | 2020-02-28T01:07:03Z | |
dc.date.issued | 2020-02-23 | |
dc.identifier.citation | Dimov , N , McDonnell , M B , Munro , I , McCluskey , D , Johnston , I , Tan , C & Coudron , L 2020 , ' Electrowetting-Based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay ' , Journal of Visualized Experiments , vol. 2020 , no. 156 , e60489 . https://doi.org/10.3791/60489 | |
dc.identifier.issn | 1940-087X | |
dc.identifier.other | ORCID: /0000-0001-9696-3191/work/69813629 | |
dc.identifier.other | ORCID: /0000-0002-2873-1505/work/69813826 | |
dc.identifier.other | ORCID: /0000-0001-9199-938X/work/113023238 | |
dc.identifier.uri | http://hdl.handle.net/2299/22356 | |
dc.description.abstract | Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to enhance the charge density and hence boost the electrowetting effect. The presence of charges results in an electrically induced spreading of the droplet which permits purposeful manipulation across a hydrophobic surface. Here, we demonstrate EWOD-based protocol for sample processing and detection of four categories of antigens, using an automated surface actuation platform, via two variations of an Enzyme-Linked Immunosorbent Assay (ELISA) methods. The ELISA is performed on magnetic beads with immobilized primary antibodies which can be selected to target a specific antigen. An antibody conjugated to HRP binds to the antigen and is mixed with H 2O 2/Luminol for quantification of the captured pathogens. Assay completion times of between 6 and 10 min were achieved, whilst minuscule volumes of reagents were utilized. | en |
dc.format.extent | 1920032 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Visualized Experiments | |
dc.subject | Automation | |
dc.subject | Biodetection | |
dc.subject | Bioengineering | |
dc.subject | Chemiluminescence | |
dc.subject | DMF | |
dc.subject | Digital microfluidics | |
dc.subject | ELISA | |
dc.subject | EWOD | |
dc.subject | Electrowetting on Dielectric | |
dc.subject | Enzyme-Linked Immunosorbent Assay | |
dc.subject | Issue 156 | |
dc.subject | Pathogen quantification | |
dc.subject | General Neuroscience | |
dc.subject | General Chemical Engineering | |
dc.subject | General Biochemistry,Genetics and Molecular Biology | |
dc.subject | General Immunology and Microbiology | |
dc.title | Electrowetting-Based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay | en |
dc.contributor.institution | Centre for Engineering Research | |
dc.contributor.institution | Centre for Research in Biodetection Technologies | |
dc.contributor.institution | Micro Electro-Mechanical Systems | |
dc.contributor.institution | Microfluidics and Microengineering | |
dc.contributor.institution | BioEngineering | |
dc.contributor.institution | School of Physics, Engineering & Computer Science | |
dc.contributor.institution | Department of Engineering and Technology | |
dc.contributor.institution | School of Engineering and Technology | |
dc.contributor.institution | SPECS Deans Group | |
dc.contributor.institution | Centre for Hazard Detection and Protection Research | |
dc.contributor.institution | Extracellular Vesicle Research Unit | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85081532579&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.3791/60489 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |