dc.contributor.author | Abdelmohsen, Shaimaa | |
dc.contributor.author | Verheecke-Vaessen, Carol | |
dc.contributor.author | Garcia-Cela, Esther | |
dc.contributor.author | Medina, Angel | |
dc.contributor.author | Magan, Naresh | |
dc.date.accessioned | 2020-04-22T00:07:14Z | |
dc.date.available | 2020-04-22T00:07:14Z | |
dc.date.issued | 2020-03-27 | |
dc.identifier.citation | Abdelmohsen , S , Verheecke-Vaessen , C , Garcia-Cela , E , Medina , A & Magan , N 2020 , ' Solute and matric potential stress on Penicillium verrucosum : Impact on growth, gene expression and ochratoxin A p production ' , World Mycotoxin Journal , vol. 13 , no. 3 , pp. 345-353 . https://doi.org/10.3920/WMJ2019.2542 | |
dc.identifier.issn | 1875-0710 | |
dc.identifier.uri | http://hdl.handle.net/2299/22624 | |
dc.description | Funding Information: S.A. is grateful to the British Council and the Newton Musharraf Programme for financial support. Publisher Copyright: © 2020 Wageningen Academic Publishers. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved. | |
dc.description.abstract | Penicillium verrucosum survives in soil and on cereal debris. It colonises grain during harvesting, drying and storage. There is no information on the relative tolerance of P. verrucosum to solute and matric stress in terms of colonisation, or on the biosynthetic toxin gene clusters or ochratoxin A (OTA) production. The objectives were to examine the effect of ionic and non-ionic solute and matric potential stress on (a) growth, (b) expression of two toxin biosynthetic genes otapksPV and otanrpsPV, and (c) OTA production by a strain of P. verrucosum. Optimum growth and OTA production were at-7.0 MPa (= 0.95 water activity, aw) and-1.4 MPa (= 0.99 aw), respectively, regardless of whether solute (Ψs) or matric (Ψm) stress was imposed. P. verrucosum was more sensitive to ionic solute stress (NaCl) with no growth at-19.6 MPa (=0.86 aw) while growth still occurred in the non-ionic solute (glycerol) and matric stress treatments. Relative gene expression of the biosynthetic genes using PCR (RT-qPCR) showed that the otapksPV gene was expressed over a wide range of ionic/non-ionic solute stress conditions (-1.4 to-14.0 MPa; = 0.99-0.90 aw). The highest expression was in the non-ionic Ψs stress treatments at-7.0 MPa (= 0.95 aw). However, the otanrpsPV gene was significantly up regulated under Ψm stress, especially with freely available water (-1.4 MPa = 0.99 aw). OTA production was significantly decreased as Ψs or Ψm stress were imposed. Limited OTA production occurred in the driest treatments under Ψs and Ψm stress respectively. The impact of these two types of stresses on the growth of P. verrucosum was quite different from that for OTA production. The results are discussed in the context of the life cycle and ecological characteristics of this species in contaminating cereals with OTA in the post-harvest phase of the cereal chain. | en |
dc.format.extent | 9 | |
dc.format.extent | 463525 | |
dc.language.iso | eng | |
dc.relation.ispartof | World Mycotoxin Journal | |
dc.subject | Biosynthetic genes | |
dc.subject | Matric potential | |
dc.subject | Mycotoxin | |
dc.subject | Ochratoxin a | |
dc.subject | Penicillium verrucosum | |
dc.subject | Qpcr | |
dc.subject | Solute potential | |
dc.subject | Water activity | |
dc.subject | Food Science | |
dc.subject | Toxicology | |
dc.subject | Public Health, Environmental and Occupational Health | |
dc.title | Solute and matric potential stress on Penicillium verrucosum : Impact on growth, gene expression and ochratoxin A p production | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Biological and Environmental Sciences | |
dc.contributor.institution | Agriculture, Food and Veterinary Sciences | |
dc.contributor.institution | Crop Protection and Climate Change | |
dc.contributor.institution | Centre for Agriculture, Food and Environmental Management Research | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85090536698&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.3920/WMJ2019.2542 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |