dc.contributor.author | Dexter, Alex | |
dc.contributor.author | Steven, Rory T | |
dc.contributor.author | Patel, Aateka | |
dc.contributor.author | Dailey, Lea Ann | |
dc.contributor.author | Taylor, Adam J | |
dc.contributor.author | Ball, Doug | |
dc.contributor.author | Klapwijk, Jan | |
dc.contributor.author | Forbes, Ben | |
dc.contributor.author | Page, Clive P | |
dc.contributor.author | Bunch, Josephine | |
dc.date.accessioned | 2020-04-25T00:33:52Z | |
dc.date.available | 2020-04-25T00:33:52Z | |
dc.date.issued | 2019-12 | |
dc.identifier.citation | Dexter , A , Steven , R T , Patel , A , Dailey , L A , Taylor , A J , Ball , D , Klapwijk , J , Forbes , B , Page , C P & Bunch , J 2019 , ' Imaging drugs, metabolites and biomarkers in rodent lung: a DESI MS strategy for the evaluation of drug-induced lipidosis ' , Analytical and Bioanalytical Chemistry , vol. 411 , no. 30 , pp. 8023-8032 . https://doi.org/10.1007/s00216-019-02151-z | |
dc.identifier.issn | 1618-2642 | |
dc.identifier.other | ORCID: /0000-0002-4172-0827/work/72783429 | |
dc.identifier.uri | http://hdl.handle.net/2299/22632 | |
dc.description | © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |
dc.description.abstract | Within drug development and pre-clinical trials, a common, significant and poorly understood event is the development of drug-induced lipidosis in tissues and cells. In this manuscript, we describe a mass spectrometry imaging strategy, involving repeated analysis of tissue sections by DESI MS, in positive and negative polarities, using MS and MS/MS modes. We present results of the detected distributions of the administered drug, drug metabolites, lipid molecules and a putative marker of lipidosis, di-docosahexaenoyl (22:6)-bis(monoacylglycerol) phosphate (di-22:6-BMP). A range of strategies have previously been reported for detection, isolation and identification of this compound, which is an isomer of di-docosahexaenoic (22:6 n-3) phosphatidylglycerol (di-22:6 PG), a commonly found lipid that acts as a surfactant in lung tissues. We show that MS imaging using MS/MS can be used to differentiate these compounds of identical mass, based upon the different distributions of abundant fragment ions. Registration of images of these fragments, and detected drugs and metabolites, is presented as a new method for studying drug-induced lipidosis in tissues. Graphical abstract. | en |
dc.format.extent | 10 | |
dc.format.extent | 3683640 | |
dc.language.iso | eng | |
dc.relation.ispartof | Analytical and Bioanalytical Chemistry | |
dc.title | Imaging drugs, metabolites and biomarkers in rodent lung: a DESI MS strategy for the evaluation of drug-induced lipidosis | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Department of Clinical and Pharmaceutical Sciences | |
dc.contributor.institution | Centre for Research into Topical Drug Delivery and Toxicology | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1007/s00216-019-02151-z | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |