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dc.contributor.authorDexter, Alex
dc.contributor.authorSteven, Rory T
dc.contributor.authorPatel, Aateka
dc.contributor.authorDailey, Lea Ann
dc.contributor.authorTaylor, Adam J
dc.contributor.authorBall, Doug
dc.contributor.authorKlapwijk, Jan
dc.contributor.authorForbes, Ben
dc.contributor.authorPage, Clive P
dc.contributor.authorBunch, Josephine
dc.date.accessioned2020-04-25T00:33:52Z
dc.date.available2020-04-25T00:33:52Z
dc.date.issued2019-12
dc.identifier.citationDexter , A , Steven , R T , Patel , A , Dailey , L A , Taylor , A J , Ball , D , Klapwijk , J , Forbes , B , Page , C P & Bunch , J 2019 , ' Imaging drugs, metabolites and biomarkers in rodent lung: a DESI MS strategy for the evaluation of drug-induced lipidosis ' , Analytical and Bioanalytical Chemistry , vol. 411 , no. 30 , pp. 8023-8032 . https://doi.org/10.1007/s00216-019-02151-z
dc.identifier.issn1618-2642
dc.identifier.otherPURE: 20976883
dc.identifier.otherPURE UUID: 64085501-bc0f-4038-8c3c-abff2c7ef171
dc.identifier.otherScopus: 85075881960
dc.identifier.otherORCID: /0000-0002-4172-0827/work/72783429
dc.identifier.urihttp://hdl.handle.net/2299/22632
dc.description© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.description.abstractWithin drug development and pre-clinical trials, a common, significant and poorly understood event is the development of drug-induced lipidosis in tissues and cells. In this manuscript, we describe a mass spectrometry imaging strategy, involving repeated analysis of tissue sections by DESI MS, in positive and negative polarities, using MS and MS/MS modes. We present results of the detected distributions of the administered drug, drug metabolites, lipid molecules and a putative marker of lipidosis, di-docosahexaenoyl (22:6)-bis(monoacylglycerol) phosphate (di-22:6-BMP). A range of strategies have previously been reported for detection, isolation and identification of this compound, which is an isomer of di-docosahexaenoic (22:6 n-3) phosphatidylglycerol (di-22:6 PG), a commonly found lipid that acts as a surfactant in lung tissues. We show that MS imaging using MS/MS can be used to differentiate these compounds of identical mass, based upon the different distributions of abundant fragment ions. Registration of images of these fragments, and detected drugs and metabolites, is presented as a new method for studying drug-induced lipidosis in tissues. Graphical abstract.en
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofAnalytical and Bioanalytical Chemistry
dc.titleImaging drugs, metabolites and biomarkers in rodent lung: a DESI MS strategy for the evaluation of drug-induced lipidosisen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Clinical and Pharmaceutical Sciences
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1007/s00216-019-02151-z
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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