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dc.contributor.authorAdebowale, Oluwawemimo
dc.contributor.authorGoh, Shan
dc.contributor.authorGood, Liam
dc.date.accessioned2020-06-20T00:07:16Z
dc.date.available2020-06-20T00:07:16Z
dc.date.issued2020-06
dc.identifier.citationAdebowale , O , Goh , S & Good , L 2020 , ' The development of species-specific antisense peptide nucleic acid method for the treatment and detection of viable Salmonella ' , Heliyon , vol. 6 , no. 6 , e04110 . https://doi.org/10.1016/j.heliyon.2020.e04110
dc.identifier.issn2405-8440
dc.identifier.otherPURE: 22084705
dc.identifier.otherPURE UUID: 94bbda64-e8b7-44fd-9ea4-c0984ce24683
dc.identifier.otherScopus: 85086101836
dc.identifier.otherORCID: /0000-0002-9028-0303/work/75948890
dc.identifier.urihttp://hdl.handle.net/2299/22895
dc.description© 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.description.abstractGenotypic based detection methods using specific target sites in the pathogen genome can complement phenotypic identification. We report the development of species-specific antisense peptide nucleic acid (PNA) combined with selective and differential enrichment growth conditions for Salmonella treatment and detection. An antisense PNA oligomer targeting the Salmonella ftsZ gene and conjugated with a cell-penetrating peptide ((KFF)3K) was exploited to probe bacteria cultured in three different growth media (Muller Hinton broth (MHB), Rappaport-Vassiliadis Soya Peptone Broth (RVS, Oxoid), and in-house modified Rappaport-Vassiliadis Soya Peptone Broths (mRVSs). Also, water and milk artificially contaminated with bacteria were probed. Antisense PNA provided detectable changes in Salmonella growth and morphology in all media and artificially contaminated matrices except RVS. Salmonella was detected as elongated cells. On the contrary, treated Escherichia coli did not elongate, providing evidence of differentiation and selectivity for Salmonella. Similarly, Salmonella probed with mismatched PNAs did not elongate. Antisense oligomers targeted ftsZ mRNA in combination with selective growth conditions can provide a detection strategy for viable Salmonella in a single reaction, and act as a potential tool for bacteria detection in real food and environmental samples.en
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofHeliyon
dc.rightsOpen
dc.titleThe development of species-specific antisense peptide nucleic acid method for the treatment and detection of viable Salmonellaen
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionExtracellular Vesicle Research Unit
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Agriculture, Food and Environmental Management Research
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
dc.description.versiontypeFinal Published version
dcterms.dateAccepted2020-06
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.heliyon.2020.e04110
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue
herts.rights.accesstypeOpen


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