dc.contributor.author | Hodeify, Rawad | |
dc.contributor.author | Siddiqui, Shoib Sarwar | |
dc.contributor.author | Matar, Rachel | |
dc.contributor.author | Vazhappilly, Cijo George | |
dc.contributor.author | Merheb, Maxime | |
dc.contributor.author | Al Zouabi, Hussain | |
dc.contributor.author | Marton, John | |
dc.date.accessioned | 2021-02-15T11:00:02Z | |
dc.date.available | 2021-02-15T11:00:02Z | |
dc.date.issued | 2021-01-22 | |
dc.identifier.citation | Hodeify , R , Siddiqui , S S , Matar , R , Vazhappilly , C G , Merheb , M , Al Zouabi , H & Marton , J 2021 , ' Modulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cells ' , Heliyon , vol. 7 , no. 1 , e06041 . https://doi.org/10.1016/j.heliyon.2021.e06041 | |
dc.identifier.issn | 2405-8440 | |
dc.identifier.other | PubMedCentral: PMC7829211 | |
dc.identifier.uri | http://hdl.handle.net/2299/23907 | |
dc.description | © 2021 The Author(s). | |
dc.description.abstract | Cisplatin (CDDP) is currently one of the most effective FDA-approved treatments for breast cancer. Previous studies have shown that CDDP-induced cell death in human breast cancer (MCF-7) cells is associated with disruption of calcium homeostasis. However, whether the sensitivity of breast cancer cells to cisplatin is associated with dysregulation of the expression of calcium-binding proteins (CaBPs) remains unknown. In this study, we evaluated the effect of the intracellular calcium chelator (BAPTA-AM) on viability of MCF-7 cells in the presence of toxic and sub-toxic doses of cisplatin. Furthermore, this study assessed the expression of CaBPs, calmodulin, S100A8, and S100A14 in MCF-7 cells treated with cisplatin. Cell viability was determined using MTT-based in vitro toxicity assay. Intracellular calcium imaging was done using Fluo-4 AM, a cell-permeant fluorescent calcium indicator. Expression of CaBPs was tested using real-time quantitative PCR. Exposure of cells to increasing amounts of CDDP correlated with increasing fluorescence of the intracellular calcium indicator, Fluo-4 AM. Conversely, treating cells with cisplatin significantly decreased mRNA levels of calmodulin, S100A8, and S100A14. Treatment of the cells with calcium chelator, BAPTA-AM, significantly enhanced the cytotoxic effects of sub-toxic dose of cisplatin. Our results indicated a statistically significant negative correlation between calmodulin, S100A8, and S100A14 expression and sensitivity of breast cancer cells to a sub-toxic dose of cisplatin. We propose that modulating the activity of calcium-binding proteins, calmodulin, S100A8, and S100A14, could be used to increase cisplatin efficacy, lowering its treatment dosage while maintaining its chemotherapeutic value. | en |
dc.format.extent | 2262050 | |
dc.language.iso | eng | |
dc.relation.ispartof | Heliyon | |
dc.subject | BAPTA-AM | |
dc.subject | Calcium-binding proteins | |
dc.subject | Cisplatin | |
dc.subject | Intracellular calcium | |
dc.subject | General | |
dc.title | Modulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cells | en |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.contributor.institution | Centre for Future Societies Research | |
dc.description.status | Peer reviewed | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85099642180&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1016/j.heliyon.2021.e06041 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |