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dc.contributor.authorHodeify, Rawad
dc.contributor.authorSiddiqui, Shoib Sarwar
dc.contributor.authorMatar, Rachel
dc.contributor.authorVazhappilly, Cijo George
dc.contributor.authorMerheb, Maxime
dc.contributor.authorAl Zouabi, Hussain
dc.contributor.authorMarton, John
dc.date.accessioned2021-02-15T11:00:02Z
dc.date.available2021-02-15T11:00:02Z
dc.date.issued2021-01-22
dc.identifier.citationHodeify , R , Siddiqui , S S , Matar , R , Vazhappilly , C G , Merheb , M , Al Zouabi , H & Marton , J 2021 , ' Modulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cells ' , Heliyon , vol. 7 , no. 1 , e06041 . https://doi.org/10.1016/j.heliyon.2021.e06041
dc.identifier.issn2405-8440
dc.identifier.otherPURE: 24635013
dc.identifier.otherPURE UUID: 36b0c16a-9a07-4775-8bc8-593c655d7bf4
dc.identifier.otherPubMed: 33532651
dc.identifier.otherPubMedCentral: PMC7829211
dc.identifier.otherScopus: 85099642180
dc.identifier.urihttp://hdl.handle.net/2299/23907
dc.description© 2021 The Author(s).
dc.description.abstractCisplatin (CDDP) is currently one of the most effective FDA-approved treatments for breast cancer. Previous studies have shown that CDDP-induced cell death in human breast cancer (MCF-7) cells is associated with disruption of calcium homeostasis. However, whether the sensitivity of breast cancer cells to cisplatin is associated with dysregulation of the expression of calcium-binding proteins (CaBPs) remains unknown. In this study, we evaluated the effect of the intracellular calcium chelator (BAPTA-AM) on viability of MCF-7 cells in the presence of toxic and sub-toxic doses of cisplatin. Furthermore, this study assessed the expression of CaBPs, calmodulin, S100A8, and S100A14 in MCF-7 cells treated with cisplatin. Cell viability was determined using MTT-based in vitro toxicity assay. Intracellular calcium imaging was done using Fluo-4 AM, a cell-permeant fluorescent calcium indicator. Expression of CaBPs was tested using real-time quantitative PCR. Exposure of cells to increasing amounts of CDDP correlated with increasing fluorescence of the intracellular calcium indicator, Fluo-4 AM. Conversely, treating cells with cisplatin significantly decreased mRNA levels of calmodulin, S100A8, and S100A14. Treatment of the cells with calcium chelator, BAPTA-AM, significantly enhanced the cytotoxic effects of sub-toxic dose of cisplatin. Our results indicated a statistically significant negative correlation between calmodulin, S100A8, and S100A14 expression and sensitivity of breast cancer cells to a sub-toxic dose of cisplatin. We propose that modulating the activity of calcium-binding proteins, calmodulin, S100A8, and S100A14, could be used to increase cisplatin efficacy, lowering its treatment dosage while maintaining its chemotherapeutic value.en
dc.language.isoeng
dc.relation.ispartofHeliyon
dc.titleModulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cellsen
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1016/j.heliyon.2021.e06041
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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