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dc.contributor.authorOrr, Jamie
dc.contributor.authorMauchline, Tim H.
dc.contributor.authorCock, Peter
dc.contributor.authorBlok, Vivian
dc.contributor.authorDavies, Keith
dc.date.accessioned2021-02-18T00:03:53Z
dc.date.available2021-02-18T00:03:53Z
dc.date.issued2018-12-04
dc.identifier.citationOrr , J , Mauchline , T H , Cock , P , Blok , V & Davies , K 2018 ' De novo assembly of the Pasteuria penetrans genome reveals high plasticity, host dependency, and BclA-like collagens ' University of Hertfordshire . < https://www.biorxiv.org/content/10.1101/485748v1 >
dc.identifier.otherPURE: 24601471
dc.identifier.otherPURE UUID: fe5a8075-46af-402b-8c02-43de196fe699
dc.identifier.otherORCID: /0000-0001-6060-2394/work/89118988
dc.identifier.urihttp://hdl.handle.net/2299/23921
dc.descriptionThis version edited from BioRxiv pre-print to remove pre-publication template marks. Otherwise undisturbed.
dc.description.abstractPasteuria penetrans is a gram-positive endospore forming bacterial parasite of Meloidogyne spp. the most economically damaging genus of plant parasitic nematodes globally. The obligate antagonistic nature of P. penetrans makes it an attractive candidate biological control agent. However, deployment of P. penetrans for this purpose is inhibited by a lack of understanding of its metabolism and the molecular mechanics underpinning parasitism of the host, in particular the initial attachment of the endospore to the nematode cuticle. Several attempts to assemble the genomes of species within this genus have been unsuccessful. Primarily this is due to the obligate parasitic nature of the bacterium which makes obtaining genomic DNA of sufficient quantity and quality which is free from contamination challenging. Taking advantage of recent developments in whole genome amplification, long read sequencing platforms, and assembly algorithms, we have developed a protocol to generate large quantities of high molecular weight genomic DNA from a small number of purified endospores. We demonstrate this method via genomic assembly of P. penetrans. This assembly reveals a reduced genome of 2.64Mbp estimated to represent 86% of the complete sequence; its reduced metabolism reflects widespread reliance on the host and possibly associated organisms. Additionally, apparent expansion of transposases and prediction of partial competence pathways suggest a high degree of genomic plasticity. Phylogenetic analysis places our sequence within the Bacilli, and most closely related to Thermoactinomyces species. Seventeen predicted BclA-like proteins are identified which may be involved in the determination of attachment specificity. This resource may be used to develop in vitro culture methods and to investigate the genetic and molecular basis of attachment specificity.en
dc.language.isoeng
dc.publisherUniversity of Hertfordshire
dc.subjectHYPERPARASITE
dc.subjectMELOIDOGYNE
dc.subjectBIOCONTROL
dc.subjectApplied Microbiology and Biotechnology
dc.titleDe novo assembly of the Pasteuria penetrans genome reveals high plasticity, host dependency, and BclA-like collagensen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop Protection and Climate Change
dc.identifier.urlhttps://www.biorxiv.org/content/10.1101/485748v1
rioxxterms.versionSMUR
rioxxterms.typeWorking paper
herts.preservation.rarelyaccessedtrue


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