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dc.contributor.authorGoh, Shan
dc.contributor.authorKolakowski, Jeannine
dc.contributor.authorHolder, Angela
dc.contributor.authorPfuhl, Mark
dc.contributor.authorNgugi, Daniel
dc.contributor.authorBallingall, Keith
dc.contributor.authorTombacz, Kata
dc.contributor.authorWerling, Dirk
dc.date.accessioned2021-07-21T09:43:24Z
dc.date.available2021-07-21T09:43:24Z
dc.date.issued2021-07-08
dc.identifier.citationGoh , S , Kolakowski , J , Holder , A , Pfuhl , M , Ngugi , D , Ballingall , K , Tombacz , K & Werling , D 2021 , ' Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle ' , Frontiers in Immunology , vol. 12 , no. July 2021 , 674484 . https://doi.org/10.3389/fimmu.2021.674484
dc.identifier.issn1664-3224
dc.identifier.otherPURE: 25550388
dc.identifier.otherPURE UUID: c6b2ecbe-cadf-4672-bd2a-04289bb2efd8
dc.identifier.otherORCID: /0000-0002-9028-0303/work/96782561
dc.identifier.otherScopus: 85111051727
dc.identifier.urihttp://hdl.handle.net/2299/24893
dc.description© 2021 Goh, Kolakowski, Holder, Pfuhl, Ngugi, Ballingall, Tombacz and Werling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). https://creativecommons.org/licenses/by/4.0/
dc.description.abstractEast Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every year. Disease prevention relies on the so called “Infection and Treatment Method” (ITM), which is costly, complex, laborious, difficult to standardise on a commercial scale and results in a parasite strain-specific, MHC class Irestricted cytotoxic T cell response. We therefore attempted to develop a safe, affordable, stable, orally applicable and potent subunit vaccine for ECF using five different T. parva schizont antigens (Tp1, Tp2, Tp9, Tp10 and N36) and Saccharomyces cerevisiae as an expression platform. Full-length Tp2 and Tp9 as well as fragments of Tp1 were successfully expressed on the surface of S. cerevisiae. In vitro analyses highlighted that recombinant yeast expressing Tp2 can elicit IFNg responses using PBMCs from ITM immunized calves, while Tp2 and Tp9 induced IFNg responses from enriched bovine CD8+ T cells. A subsequent in vivo study showed that oral administration of heat inactivated, freeze-dried yeast stably expressing Tp2 increased total murine serum IgG over time, but more importantly, induced Tp2-specific serum IgG antibodies in individual mice compared to the control group. While these results will require subsequent experiments to verify induction of protection in neonatal calves, our data indicates that oral application of yeast expressing Theileria antigens could provide an affordable and easy vaccination platform for sub-Saharan Africa. Evaluation of antigen-specific cellular immune responses, especially cytotoxic CD8+ T cell immunity in cattle will further contribute to the development of a yeast-based vaccine for ECF.en
dc.format.extent18
dc.language.isoeng
dc.relation.ispartofFrontiers in Immunology
dc.subjectTheileria parva
dc.subjectEast Coast Fever
dc.subjectschizont antigens
dc.subjectoral vaccine
dc.subjectyeast
dc.titleDevelopment of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattleen
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionExtracellular Vesicle Research Unit
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Agriculture, Food and Environmental Management Research
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.3389/fimmu.2021.674484
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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