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dc.contributor.authorWalsh, Claire
dc.contributor.authorHolroyd, Natalie
dc.contributor.authorFinnerty, Eoin
dc.contributor.authorRyan, Sean
dc.contributor.authorSweeney, Paul
dc.contributor.authorShipley, Rebecca
dc.contributor.authorWalker-Samuel, Simon
dc.date.accessioned2021-08-05T13:30:02Z
dc.date.available2021-08-05T13:30:02Z
dc.date.issued2021-05-26
dc.identifier.citationWalsh , C , Holroyd , N , Finnerty , E , Ryan , S , Sweeney , P , Shipley , R & Walker-Samuel , S 2021 , ' Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs ' , Advanced Photonics Research . https://doi.org/10.1002/adpr.202100110
dc.identifier.otherORCID: /0000-0001-9069-5122/work/98164015
dc.identifier.urihttp://hdl.handle.net/2299/24955
dc.description© 2021 The Authors. Advanced Photonics Research published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, https://creativecommons.org/licenses/by/4.0/
dc.description.abstractThree-dimensional microscopy of large biological samples (greater than 0.5 cm^3) is transforming biological research. Many existing techniques require trade-offs between image resolution, sample size and method complexity. A simple robust instrument with the potential to perform large volume 3D imaging currently exists in the form of the Optical HREM, however the development of the instrument to date is limited to single fluorescent wavelength imaging with non-specific eosin staining. This work presents developments to realize the potential of the HREM to become Multi-fluorescent High Resolution Episcopic Microscopy (MF-HREM). MF-HREM is a serial-sectioning and block-facing wide-field fluorescence imaging technique, which does not require tissue clearing or optical sectioning. Multiple developments are detailed in sample preparation and image post-processing to enable multiple specific stains in large samples, and show how these enable segmentation and quantification of the data. The application of MF-HREM is demonstrated in a variety of biological contexts: 3D imaging of whole tumor vascular networks and tumor cell invasion in xenograft tumors up to 7.5 mm^3 at resolutions of 2.75 mm, quantification of glomeruli volume in the adult mouse kidney, and quantification of vascular networks and white matter track orientation in adult mouse brain.en
dc.format.extent13
dc.format.extent3173626
dc.language.isoeng
dc.relation.ispartofAdvanced Photonics Research
dc.subjectserial-sectioning
dc.subjectHREM
dc.subjectdeconvolution
dc.subjecttumor
dc.subjectwhole-mount
dc.subjectBiochemistry
dc.subjectBiotechnology
dc.subjectPhysiology
dc.subjectBiochemistry, medical
dc.subjectPhysiology (medical)
dc.titleMulti-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organsen
dc.contributor.institutionSchool of Physics, Engineering & Computer Science
dc.contributor.institutionCentre for Astrophysics Research
dc.contributor.institutionDepartment of Physics, Astronomy and Mathematics
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1002/adpr.202100110
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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