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dc.contributor.authorSheelan, Ahmad,
dc.contributor.authorBaker, Daniel
dc.contributor.authorMurnane, Darragh
dc.contributor.authorNeil, Spooner,
dc.contributor.authorGerhard, Ute
dc.date.accessioned2021-08-23T14:30:03Z
dc.date.available2021-08-23T14:30:03Z
dc.date.issued2021-07-19
dc.identifier.citationSheelan , A , Baker , D , Murnane , D , Neil , S & Gerhard , U 2021 , ' Solid-phase microextraction for assessment of plasma protein binding, a complement to rapid equilibrium dialysis ' , Bioanalysis , vol. 13 , no. 14 , pp. 1101-1111 . https://doi.org/10.4155/bio-2021-0109
dc.identifier.issn1757-6180
dc.identifier.otherPURE: 25480115
dc.identifier.otherPURE UUID: 48ab0d34-6c4a-4b70-8fb3-2178ee474b51
dc.identifier.otherJisc: b39acad4175d4207b7a597cf5920d6a3
dc.identifier.otherJisc: b39acad4175d4207b7a597cf5920d6a3
dc.identifier.otherScopus: 85111147987
dc.identifier.urihttp://hdl.handle.net/2299/24999
dc.description© 2021 Future Science Group.
dc.description.abstractAim: Determination of plasma protein binding (PPB) is considered vital for better understanding of pharmacokinetic and pharmacodynamic activities of drugs due to the role of free concentration in pharmacological response.  Methodology & results: Solid-phase microextraction (SPME) was investigated for measurement of PPB from biological matrices and compared with a gold standard approach (rapid equilibrium dialysis [RED]).  Discussion & conclusion: SPME-derived values of PPB correlated well with literature values, and those determined by RED. Respectively, average protein binding across three concentrations by RED and SPME was 33.1 and 31.7% for metoprolol, 89.0 and 86.6% for propranolol and 99.2 and 99.0% for diclofenac. This study generates some evidence for SPME as an alternative platform for the determination of PPB.en
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofBioanalysis
dc.subjectPreliminary Communication
dc.subjectfree concentration
dc.subjectmicrosampling
dc.subjectplasma protein binding
dc.subjectrapid equilibrium dialysis
dc.subjectsolid-phase microextraction
dc.subjectAnalytical Chemistry
dc.subjectMedical Laboratory Technology
dc.subjectPharmacology, Toxicology and Pharmaceutics(all)
dc.subjectClinical Biochemistry
dc.titleSolid-phase microextraction for assessment of plasma protein binding, a complement to rapid equilibrium dialysisen
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionAirway Group
dc.contributor.institutionPharmaceutics
dc.contributor.institutionPharmaceutical Analysis and Product Characterisation
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.description.statusPeer reviewed
dc.date.embargoedUntil2022-07-19
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85111147987&partnerID=8YFLogxK
dc.relation.schoolSchool of Life and Medical Sciences
dcterms.dateAccepted2021-07-19
rioxxterms.versionAM
rioxxterms.versionofrecordhttps://doi.org/10.4155/bio-2021-0109
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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