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dc.contributor.authorMensah, Rosemond A
dc.contributor.authorJo, Seung Bin
dc.contributor.authorKim, Hoon
dc.contributor.authorPark, Sung-Min
dc.contributor.authorPatel, Kapil D
dc.contributor.authorCho, Kyong J
dc.contributor.authorCook, Michael T
dc.contributor.authorKirton, Stewart B
dc.contributor.authorHutter, Victoria
dc.contributor.authorSidney, Laura E
dc.contributor.authorAlves-Lima, Decio
dc.contributor.authorLin, Hungyen
dc.contributor.authorLee, Jung-Hwan
dc.contributor.authorKim, Hae-Won
dc.contributor.authorChau, David Ys
dc.date.accessioned2021-08-24T13:30:01Z
dc.date.available2021-08-24T13:30:01Z
dc.date.issued2021-06-18
dc.identifier.citationMensah , R A , Jo , S B , Kim , H , Park , S-M , Patel , K D , Cho , K J , Cook , M T , Kirton , S B , Hutter , V , Sidney , L E , Alves-Lima , D , Lin , H , Lee , J-H , Kim , H-W & Chau , D Y 2021 , ' The eggshell membrane : A potential biomaterial for corneal wound healing ' , Journal of biomaterials applications , pp. 8853282211024040 . https://doi.org/10.1177/08853282211024040
dc.identifier.issn0885-3282
dc.identifier.otherPURE: 25810991
dc.identifier.otherPURE UUID: 24ef6124-d821-4ef7-8bdd-f892c2ec3181
dc.identifier.otherPubMed: 34139891
dc.identifier.otherScopus: 85108578212
dc.identifier.urihttp://hdl.handle.net/2299/25012
dc.description.abstractThe eggshell membrane (ESM) is an abundant resource with innate complex structure and composition provided by nature. With at least 60 million tonnes of hen eggs produced globally per annum, utilisation of this waste resource is highly attractive in positively impacting sustainability worldwide. Given the morphology and mechanical properties of this membrane, it has great potential as a biomaterials for wound dressing. However, to date, no studies have demonstrated nor reported this application. As such, the objective of this investigation was to identify and optimise a reproducible extraction protocol of the ESM and to assess the physical, chemical, mechanical and biological properties of the substrate with a view to use as a wound dressing. ESM samples were isolated by either manual peeling (ESM-strip) or via extraction using acetic acid [ESM-A0.5] or ethylenediaminetetraacetic acid, EDTA [ESM-E0.9]. Energy dispersive X-ray spectroscopy (EDS) confirmed that there were no traces of calcium residues from the extraction process. Fourier transform infrared (FTIR) spectroscopy revealed that the extraction method (acetic acid and EDTA) did not alter the chemical structures of the ESM and also clarified the composition of the fibrous proteins of the ESM. Scanning electron microscopy (SEM) analyses revealed a three-layer composite structure of the ESM: an inner layer as continuous, dense and non-fibrous (limiting membrane), a middle layer with a network of fibres (inner shell membrane) and the outer layer (outer shell membrane) of larger fibres. Material properties including optical transparency, porosity, fluid absorption/uptake, thermal stability, mechanical profiling of the ESM samples were performed and demonstrated suitable profiles for translational applications. Biological in vitro studies using SV40 immortalised corneal epithelial cells (ihCEC) and corneal mesenchymal stromal cells (C-MSC) demonstrated excellent biocompatibility. Taken together, these results document the development of a novel sustainable biomaterial that may be used for ophthalmic wounds and/or other biomedical therapies.en
dc.language.isoeng
dc.relation.ispartofJournal of biomaterials applications
dc.subjectHealth and Wellbeing
dc.titleThe eggshell membrane : A potential biomaterial for corneal wound healingen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Research into Topical Drug Delivery and Toxicology
dc.contributor.institutionDepartment of Clinical and Pharmaceutical Sciences
dc.contributor.institutionDepartment of Pharmacy, Pharmacology and Postgraduate Medicine
dc.contributor.institutionCentre for Health Services and Clinical Research
dc.contributor.institutionPsychopharmacology, Drug Misuse and Novel Psychoactive Substances Unit
dc.contributor.institutionNatural Product Chemistry and Drug Design
dc.contributor.institutionAirway Group
dc.contributor.institutionPharmaceutics
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1177/08853282211024040
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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